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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 50S ribosomal protein L11 (rplK) CSB-YP772812SYG
CSB-EP772812SYG
CSB-BP772812SYG
CSB-MP772812SYG
CSB-EP772812SYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UPF0725 protein At2g19200 (At2g19200) CSB-YP772813DOA
CSB-EP772813DOA
CSB-BP772813DOA
CSB-MP772813DOA
CSB-EP772813DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Single-stranded DNA-binding protein, mitochondrial (At4g11060), partial CSB-YP772814DOA
CSB-EP772814DOA
CSB-BP772814DOA
CSB-MP772814DOA
CSB-EP772814DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 30 (Os03g0268600, LOC_Os03g16170) CSB-YP772815OFG
CSB-EP772815OFG
CSB-BP772815OFG
CSB-MP772815OFG
CSB-EP772815OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana TPR repeat-containing thioredoxin TTL4 (TTL4), partial CSB-YP772816DOA
CSB-EP772816DOA
CSB-BP772816DOA
CSB-MP772816DOA
CSB-EP772816DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Serine/threonine-protein phosphatase 5 (PP5), partial CSB-YP772817SZY1
CSB-EP772817SZY1
CSB-BP772817SZY1
CSB-MP772817SZY1
CSB-EP772817SZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cryptochrome DASH, chloroplastic/mitochondrial (CRYD), partial CSB-YP772818DOA
CSB-EP772818DOA
CSB-BP772818DOA
CSB-MP772818DOA
CSB-EP772818DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDP-mannose transporter GONST2 (GONST2), partial CSB-YP772819DOA
CSB-EP772819DOA
CSB-BP772819DOA
CSB-MP772819DOA
CSB-EP772819DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malus domestica (E,E)-alpha-farnesene synthase (AFS1), partial CSB-YP772820MPS
CSB-EP772820MPS
CSB-BP772820MPS
CSB-MP772820MPS
CSB-EP772820MPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor PIF5 (PIF5) CSB-YP772821DOA
CSB-EP772821DOA
CSB-BP772821DOA
CSB-MP772821DOA
CSB-EP772821DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable cytokinin riboside 5'-monophosphate phosphoribohydrolase LOGL5 (LOGL5) CSB-YP772822OFG
CSB-EP772822OFG
CSB-BP772822OFG
CSB-MP772822OFG
CSB-EP772822OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Adenine/guanine permease AZG2 (AZG2), partial CSB-YP772823DOA
CSB-EP772823DOA
CSB-BP772823DOA
CSB-MP772823DOA
CSB-EP772823DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Abscisic acid receptor PYL9 (PYL9) CSB-YP772824DOA
CSB-EP772824DOA
CSB-BP772824DOA
CSB-MP772824DOA
CSB-EP772824DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable histone H2A variant 3 (Os03g0743400, LOC_Os03g53190) CSB-YP772825OFG
CSB-EP772825OFG
CSB-BP772825OFG
CSB-MP772825OFG
CSB-EP772825OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant OBERON-like protein (PVIP), partial CSB-YP772826NDG
CSB-EP772826NDG
CSB-BP772826NDG
CSB-MP772826NDG
CSB-EP772826NDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Thioredoxin-like protein CDSP32, chloroplastic (CDSP32) CSB-YP772827OFG
CSB-EP772827OFG
CSB-BP772827OFG
CSB-MP772827OFG
CSB-EP772827OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 4-coumarate--CoA ligase-like 2 (4CLL2), partial CSB-YP772828DOA
CSB-EP772828DOA
CSB-BP772828DOA
CSB-MP772828DOA
CSB-EP772828DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coffea arabica Monomethylxanthine methyltransferase 2 (MXMT2) CSB-YP772829DVA
CSB-EP772829DVA
CSB-BP772829DVA
CSB-MP772829DVA
CSB-EP772829DVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 12-oxophytodienoate reductase 1 (OPR1) CSB-YP772830OFG
CSB-EP772830OFG
CSB-BP772830OFG
CSB-MP772830OFG
CSB-EP772830OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At2g44700 (At2g44700) CSB-YP772831DOA
CSB-EP772831DOA
CSB-BP772831DOA
CSB-MP772831DOA
CSB-EP772831DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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