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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas syringae pv. tomato Urocanate hydratase (hutU), partial CSB-YP771678FGP
CSB-EP771678FGP
CSB-BP771678FGP
CSB-MP771678FGP
CSB-EP771678FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Na (+)/H (+) antiporter nhaA 2 (nhaA2), partial CSB-YP771679FGP
CSB-EP771679FGP
CSB-BP771679FGP
CSB-MP771679FGP
CSB-EP771679FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP771680FGP
CSB-EP771680FGP
CSB-BP771680FGP
CSB-MP771680FGP
CSB-EP771680FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Non-canonical purine NTP pyrophosphatase (PSPTO_5051) CSB-YP771681FGP
CSB-EP771681FGP
CSB-BP771681FGP
CSB-MP771681FGP
CSB-EP771681FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphomethylpyrimidine synthase (thiC), partial CSB-YP771682FGP
CSB-EP771682FGP
CSB-BP771682FGP
CSB-MP771682FGP
CSB-EP771682FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP771683FGP
CSB-EP771683FGP
CSB-BP771683FGP
CSB-MP771683FGP
CSB-EP771683FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 50S ribosomal protein L9 (rplI) CSB-YP771684FGP
CSB-EP771684FGP
CSB-BP771684FGP
CSB-MP771684FGP
CSB-EP771684FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Curved DNA-binding protein (cbpA) CSB-YP771685FGP
CSB-EP771685FGP
CSB-BP771685FGP
CSB-MP771685FGP
CSB-EP771685FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP771686FGP
CSB-EP771686FGP
CSB-BP771686FGP
CSB-MP771686FGP
CSB-EP771686FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Putative 3-methyladenine DNA glycosylase (PSPTO_4830) CSB-YP771687FGP
CSB-EP771687FGP
CSB-BP771687FGP
CSB-MP771687FGP
CSB-EP771687FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0250 protein PSPTO_4820 (PSPTO_4820) CSB-YP771688FGP
CSB-EP771688FGP
CSB-BP771688FGP
CSB-MP771688FGP
CSB-EP771688FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP771689FGP
CSB-EP771689FGP
CSB-BP771689FGP
CSB-MP771689FGP
CSB-EP771689FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0114 protein PSPTO_4583 (PSPTO_4583), partial CSB-YP771690FGP1
CSB-EP771690FGP1
CSB-BP771690FGP1
CSB-MP771690FGP1
CSB-EP771690FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato SsrA-binding protein (smpB) CSB-YP771691FGP
CSB-EP771691FGP
CSB-BP771691FGP
CSB-MP771691FGP
CSB-EP771691FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Carbamoyl-phosphate synthase small chain (carA) CSB-YP771692FGP
CSB-EP771692FGP
CSB-BP771692FGP
CSB-MP771692FGP
CSB-EP771692FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Triosephosphate isomerase (tpiA) CSB-YP771693FGP
CSB-EP771693FGP
CSB-BP771693FGP
CSB-MP771693FGP
CSB-EP771693FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP771694FGP
CSB-EP771694FGP
CSB-BP771694FGP
CSB-MP771694FGP
CSB-EP771694FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP771695FGP
CSB-EP771695FGP
CSB-BP771695FGP
CSB-MP771695FGP
CSB-EP771695FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP771696FGP
CSB-EP771696FGP
CSB-BP771696FGP
CSB-MP771696FGP
CSB-EP771696FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato D-alanine--D-alanine ligase B (ddlB) CSB-YP771697FGP
CSB-EP771697FGP
CSB-BP771697FGP
CSB-MP771697FGP
CSB-EP771697FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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