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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter calcoaceticus Phenol hydroxylase P5 protein (mphP) CSB-YP773690AWS
CSB-EP773690AWS
CSB-BP773690AWS
CSB-MP773690AWS
CSB-EP773690AWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae Aquaporin Z (aqpZ), partial CSB-YP773691FAAX1
CSB-EP773691FAAX1
CSB-BP773691FAAX1
CSB-MP773691FAAX1
CSB-EP773691FAAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Bacteriocin-like protein sboX (sboX) CSB-YP773692BRJ
CSB-EP773692BRJ
CSB-BP773692BRJ
CSB-MP773692BRJ
CSB-EP773692BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0297 protein yrzL (yrzL) CSB-YP773693BRJ
CSB-EP773693BRJ
CSB-BP773693BRJ
CSB-MP773693BRJ
CSB-EP773693BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0295 protein ygzB (ygzB), partial CSB-YP773694BRJ1
CSB-EP773694BRJ1
CSB-BP773694BRJ1
CSB-MP773694BRJ1
CSB-EP773694BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium beijerinckii Putative AgrB-like protein (cfg02), partial CSB-YP773695CLP1
CSB-EP773695CLP1
CSB-BP773695CLP1
CSB-MP773695CLP1
CSB-EP773695CLP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33 (rpmG) CSB-YP773696FLV
CSB-EP773696FLV
CSB-BP773696FLV
CSB-MP773696FLV
CSB-EP773696FLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Carbamate kinase 2 (arcC2) CSB-YP773697FLF
CSB-EP773697FLF
CSB-BP773697FLF
CSB-MP773697FLF
CSB-EP773697FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Ribonuclease P protein component (rnpA) CSB-YP773698TNT
CSB-EP773698TNT
CSB-BP773698TNT
CSB-MP773698TNT
CSB-EP773698TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L34 (rpmH) CSB-YP773699TDAH
CSB-EP773699TDAH
CSB-BP773699TDAH
CSB-MP773699TDAH
CSB-EP773699TDAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g55630 (At1g55630) CSB-YP773700DOA
CSB-EP773700DOA
CSB-BP773700DOA
CSB-MP773700DOA
CSB-EP773700DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cytochrome P450 99A2 (CYP99A2), partial CSB-YP773701OFG1
CSB-EP773701OFG1
CSB-BP773701OFG1
CSB-MP773701OFG1
CSB-EP773701OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Larrea tridentata Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic (RCA2) CSB-YP773702LDAA
CSB-EP773702LDAA
CSB-BP773702LDAA
CSB-MP773702LDAA
CSB-EP773702LDAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum bulbocastanum Putative disease resistance protein RGA4 (RGA4), partial CSB-YP773703STW
CSB-EP773703STW
CSB-BP773703STW
CSB-MP773703STW
CSB-EP773703STW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter G family member 21 (ABCG21), partial CSB-YP773704DOA1
CSB-EP773704DOA1
CSB-BP773704DOA1
CSB-MP773704DOA1
CSB-EP773704DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica F-box protein GID2 (GID2) CSB-YP773705OFG
CSB-EP773705OFG
CSB-BP773705OFG
CSB-MP773705OFG
CSB-EP773705OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Calcineurin B-like protein 1 (CBL1) CSB-YP773706OFG
CSB-EP773706OFG
CSB-BP773706OFG
CSB-MP773706OFG
CSB-EP773706OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative ripening-related protein 7 (Os10g0489400, LOC_Os10g34770) CSB-YP773707OFG
CSB-EP773707OFG
CSB-BP773707OFG
CSB-MP773707OFG
CSB-EP773707OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative expansin-A27 (EXPA27) CSB-YP773708OFG
CSB-EP773708OFG
CSB-BP773708OFG
CSB-MP773708OFG
CSB-EP773708OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Beta-galactosidase 14 (Os10g0340600, LOC_Os10g19960), partial CSB-YP773709OFG
CSB-EP773709OFG
CSB-BP773709OFG
CSB-MP773709OFG
CSB-EP773709OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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