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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP772332BQE
CSB-EP772332BQE
CSB-BP772332BQE
CSB-MP772332BQE
CSB-EP772332BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP772333BQE
CSB-EP772333BQE
CSB-BP772333BQE
CSB-MP772333BQE
CSB-EP772333BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--poly (phosphoribitol) ligase subunit 1 (dltA), partial CSB-YP772334BQE
CSB-EP772334BQE
CSB-BP772334BQE
CSB-MP772334BQE
CSB-EP772334BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactate utilization protein A 1 (lutA1) CSB-YP772335BQE
CSB-EP772335BQE
CSB-BP772335BQE
CSB-MP772335BQE
CSB-EP772335BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0738 protein BA_1211/GBAA_1211/BAS1118 (BA_1211, GBAA_1211, BAS1118) CSB-YP772336BQE
CSB-EP772336BQE
CSB-BP772336BQE
CSB-MP772336BQE
CSB-EP772336BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0736 protein BA_1187/GBAA_1187/BAS1098 (BA_1187, GBAA_1187, BAS1098) CSB-YP772337BQE
CSB-EP772337BQE
CSB-BP772337BQE
CSB-MP772337BQE
CSB-EP772337BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithine aminotransferase (rocD) CSB-YP772338BQE
CSB-EP772338BQE
CSB-BP772338BQE
CSB-MP772338BQE
CSB-EP772338BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3'-5' exoribonuclease yhaM (yhaM) CSB-YP772339BQE
CSB-EP772339BQE
CSB-BP772339BQE
CSB-MP772339BQE
CSB-EP772339BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0342 protein BA_0863/GBAA_0863/BAS0820 (BA_0863, GBAA_0863, BAS0820) CSB-YP772340BQE
CSB-EP772340BQE
CSB-BP772340BQE
CSB-MP772340BQE
CSB-EP772340BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potassium-transporting ATPase A chain (kdpA), partial CSB-YP772341BQE
CSB-EP772341BQE
CSB-BP772341BQE
CSB-MP772341BQE
CSB-EP772341BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative pyridoxal phosphate-dependent acyltransferase (BA_0620, GBAA_0620, BAS0586) CSB-YP772342BQE
CSB-EP772342BQE
CSB-BP772342BQE
CSB-MP772342BQE
CSB-EP772342BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DEAD-box ATP-dependent RNA helicase CshA (cshA), partial CSB-YP772343BQE
CSB-EP772343BQE
CSB-BP772343BQE
CSB-MP772343BQE
CSB-EP772343BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L13 (rplM) CSB-YP772344BQE
CSB-EP772344BQE
CSB-BP772344BQE
CSB-MP772344BQE
CSB-EP772344BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L36 (rpmJ) CSB-YP772345BQE
CSB-EP772345BQE
CSB-BP772345BQE
CSB-MP772345BQE
CSB-EP772345BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S5 (rpsE) CSB-YP772346BQE
CSB-EP772346BQE
CSB-BP772346BQE
CSB-MP772346BQE
CSB-EP772346BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP772347BQE
CSB-EP772347BQE
CSB-BP772347BQE
CSB-MP772347BQE
CSB-EP772347BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L3 (rplC) CSB-YP772348BQE
CSB-EP772348BQE
CSB-BP772348BQE
CSB-MP772348BQE
CSB-EP772348BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L10 (rplJ) CSB-YP772349BQE
CSB-EP772349BQE
CSB-BP772349BQE
CSB-MP772349BQE
CSB-EP772349BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP772350BQE
CSB-EP772350BQE
CSB-BP772350BQE
CSB-MP772350BQE
CSB-EP772350BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type III pantothenate kinase (coaX) CSB-YP772351BQE
CSB-EP772351BQE
CSB-BP772351BQE
CSB-MP772351BQE
CSB-EP772351BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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