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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida Glycine cleavage system H protein 2 (gcvH2) CSB-YP773352FGB
CSB-EP773352FGB
CSB-BP773352FGB
CSB-MP773352FGB
CSB-EP773352FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP773353FGB
CSB-EP773353FGB
CSB-BP773353FGB
CSB-MP773353FGB
CSB-EP773353FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Homoserine O-acetyltransferase (metX) CSB-YP773354FGB
CSB-EP773354FGB
CSB-BP773354FGB
CSB-MP773354FGB
CSB-EP773354FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Betaine aldehyde dehydrogenase (betB) CSB-YP773355FGB
CSB-EP773355FGB
CSB-BP773355FGB
CSB-MP773355FGB
CSB-EP773355FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP773356FGB
CSB-EP773356FGB
CSB-BP773356FGB
CSB-MP773356FGB
CSB-EP773356FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphomethylpyrimidine synthase (thiC), partial CSB-YP773357FGB
CSB-EP773357FGB
CSB-BP773357FGB
CSB-MP773357FGB
CSB-EP773357FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA dimethylallyltransferase (miaA) CSB-YP773358FGB
CSB-EP773358FGB
CSB-BP773358FGB
CSB-MP773358FGB
CSB-EP773358FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L9 (rplI) CSB-YP773359FGB
CSB-EP773359FGB
CSB-BP773359FGB
CSB-MP773359FGB
CSB-EP773359FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Curved DNA-binding protein (cbpA) CSB-YP773360FGB
CSB-EP773360FGB
CSB-BP773360FGB
CSB-MP773360FGB
CSB-EP773360FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal protein L11 methyltransferase (prmA) CSB-YP773361FGB
CSB-EP773361FGB
CSB-BP773361FGB
CSB-MP773361FGB
CSB-EP773361FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Lipoyl synthase (lipA) CSB-YP773362FGB
CSB-EP773362FGB
CSB-BP773362FGB
CSB-MP773362FGB
CSB-EP773362FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida SsrA-binding protein (smpB) CSB-YP773363FGB
CSB-EP773363FGB
CSB-BP773363FGB
CSB-MP773363FGB
CSB-EP773363FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Transcription elongation factor GreA (greA) CSB-YP773364FGB
CSB-EP773364FGB
CSB-BP773364FGB
CSB-MP773364FGB
CSB-EP773364FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Translation initiation factor IF-2 (infB), partial CSB-YP773365FGB
CSB-EP773365FGB
CSB-BP773365FGB
CSB-MP773365FGB
CSB-EP773365FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP773366FGB
CSB-EP773366FGB
CSB-BP773366FGB
CSB-MP773366FGB
CSB-EP773366FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP773367FGB
CSB-EP773367FGB
CSB-BP773367FGB
CSB-MP773367FGB
CSB-EP773367FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0260 protein PP_4587 (PP_4587) CSB-YP773368FGB
CSB-EP773368FGB
CSB-BP773368FGB
CSB-MP773368FGB
CSB-EP773368FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida N-succinylarginine dihydrolase (astB) CSB-YP773369FGB
CSB-EP773369FGB
CSB-BP773369FGB
CSB-MP773369FGB
CSB-EP773369FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein phosphatase CheZ (cheZ) CSB-YP773370FGB
CSB-EP773370FGB
CSB-BP773370FGB
CSB-MP773370FGB
CSB-EP773370FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chaperone protein htpG (htpG), partial CSB-YP773371FGB
CSB-EP773371FGB
CSB-BP773371FGB
CSB-MP773371FGB
CSB-EP773371FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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