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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida Cobalamin synthase (cobS), partial CSB-YP773392FGB1
CSB-EP773392FGB1
CSB-BP773392FGB1
CSB-MP773392FGB1
CSB-EP773392FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein RecA (recA) CSB-YP773393FGB
CSB-EP773393FGB
CSB-BP773393FGB
CSB-MP773393FGB
CSB-EP773393FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Enolase (eno) CSB-YP773394FGB
CSB-EP773394FGB
CSB-BP773394FGB
CSB-MP773394FGB
CSB-EP773394FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP773395FGB
CSB-EP773395FGB
CSB-BP773395FGB
CSB-MP773395FGB
CSB-EP773395FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S2 (rpsB) CSB-YP773396FGB
CSB-EP773396FGB
CSB-BP773396FGB
CSB-MP773396FGB
CSB-EP773396FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Lysine--tRNA ligase (lysS) CSB-YP773397FGB
CSB-EP773397FGB
CSB-BP773397FGB
CSB-MP773397FGB
CSB-EP773397FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP773398FGB
CSB-EP773398FGB
CSB-BP773398FGB
CSB-MP773398FGB
CSB-EP773398FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Oxaloacetate decarboxylase (PP_1389) CSB-YP773399FGB
CSB-EP773399FGB
CSB-BP773399FGB
CSB-MP773399FGB
CSB-EP773399FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 10 kDa chaperonin (groS) CSB-YP773400FGB
CSB-EP773400FGB
CSB-BP773400FGB
CSB-MP773400FGB
CSB-EP773400FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP773401FGB
CSB-EP773401FGB
CSB-BP773401FGB
CSB-MP773401FGB
CSB-EP773401FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Tryptophan--tRNA ligase (trpS) CSB-YP773402FGB
CSB-EP773402FGB
CSB-BP773402FGB
CSB-MP773402FGB
CSB-EP773402FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glycosyltransferase alg8 (alg8), partial CSB-YP773403FGB1
CSB-EP773403FGB1
CSB-BP773403FGB1
CSB-MP773403FGB1
CSB-EP773403FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Alginate biosynthesis protein AlgX (algX) CSB-YP773404FGB
CSB-EP773404FGB
CSB-BP773404FGB
CSB-MP773404FGB
CSB-EP773404FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Alginate biosynthesis protein AlgA (algA) CSB-YP773405FGB
CSB-EP773405FGB
CSB-BP773405FGB
CSB-MP773405FGB
CSB-EP773405FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Aspartate--tRNA ligase (aspS), partial CSB-YP773406FGB
CSB-EP773406FGB
CSB-BP773406FGB
CSB-MP773406FGB
CSB-EP773406FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP773407FGB
CSB-EP773407FGB
CSB-BP773407FGB
CSB-MP773407FGB
CSB-EP773407FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Deoxycytidine triphosphate deaminase (dcd) CSB-YP773408FGB
CSB-EP773408FGB
CSB-BP773408FGB
CSB-MP773408FGB
CSB-EP773408FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 2-isopropylmalate synthase (leuA), partial CSB-YP773409FGB
CSB-EP773409FGB
CSB-BP773409FGB
CSB-MP773409FGB
CSB-EP773409FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glycine dehydrogenase [decarboxylating] 1 (gcvP1), partial CSB-YP773410FGB
CSB-EP773410FGB
CSB-BP773410FGB
CSB-MP773410FGB
CSB-EP773410FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0042 nucleotide-binding protein PP_0949 (PP_0949) CSB-YP773411FGB
CSB-EP773411FGB
CSB-BP773411FGB
CSB-MP773411FGB
CSB-EP773411FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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