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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas syringae pv. tomato ATP synthase subunit c (atpE), partial CSB-YP775033FGP1
CSB-EP775033FGP1
CSB-BP775033FGP1
CSB-MP775033FGP1
CSB-EP775033FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato ATP synthase epsilon chain (atpC) CSB-YP775034FGP
CSB-EP775034FGP
CSB-BP775034FGP
CSB-MP775034FGP
CSB-EP775034FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP775035FGP
CSB-EP775035FGP
CSB-BP775035FGP
CSB-MP775035FGP
CSB-EP775035FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0317 protein PSPTO_5379 (PSPTO_5379) CSB-YP775036FGP
CSB-EP775036FGP
CSB-BP775036FGP
CSB-MP775036FGP
CSB-EP775036FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Aliphatic sulfonates import ATP-binding protein SsuB 3 (ssuB3) CSB-YP775037FGP
CSB-EP775037FGP
CSB-BP775037FGP
CSB-MP775037FGP
CSB-EP775037FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Zinc import ATP-binding protein ZnuC (znuC) CSB-YP775038FGP
CSB-EP775038FGP
CSB-BP775038FGP
CSB-MP775038FGP
CSB-EP775038FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP775039FGP
CSB-EP775039FGP
CSB-BP775039FGP
CSB-MP775039FGP
CSB-EP775039FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP775040FGP1
CSB-EP775040FGP1
CSB-BP775040FGP1
CSB-MP775040FGP1
CSB-EP775040FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Shikimate kinase (aroK) CSB-YP775041FGP
CSB-EP775041FGP
CSB-BP775041FGP
CSB-MP775041FGP
CSB-EP775041FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphoribosyl-dephospho-CoA transferase (mdcG) CSB-YP775042FGP
CSB-EP775042FGP
CSB-BP775042FGP
CSB-MP775042FGP
CSB-EP775042FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Bifunctional protein pyrR (pyrR) CSB-YP775043FGP
CSB-EP775043FGP
CSB-BP775043FGP
CSB-MP775043FGP
CSB-EP775043FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0061 protein PSPTO_5028 (PSPTO_5028) CSB-YP775044FGP
CSB-EP775044FGP
CSB-BP775044FGP
CSB-MP775044FGP
CSB-EP775044FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato tRNA dimethylallyltransferase (miaA) CSB-YP775045FGP
CSB-EP775045FGP
CSB-BP775045FGP
CSB-MP775045FGP
CSB-EP775045FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 30S ribosomal protein S18 (rpsR) CSB-YP775046FGP
CSB-EP775046FGP
CSB-BP775046FGP
CSB-MP775046FGP
CSB-EP775046FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Urease accessory protein UreF 2 (ureF2) CSB-YP775047FGP
CSB-EP775047FGP
CSB-BP775047FGP
CSB-MP775047FGP
CSB-EP775047FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphoribosylamine--glycine ligase (purD) CSB-YP775048FGP
CSB-EP775048FGP
CSB-BP775048FGP
CSB-MP775048FGP
CSB-EP775048FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 3-dehydroquinate dehydratase (aroQ) CSB-YP775049FGP
CSB-EP775049FGP
CSB-BP775049FGP
CSB-MP775049FGP
CSB-EP775049FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Apolipoprotein N-acyltransferase (lnt), partial CSB-YP775050FGP1
CSB-EP775050FGP1
CSB-BP775050FGP1
CSB-MP775050FGP1
CSB-EP775050FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato NAD-dependent protein deacylase 4 (cobB4) CSB-YP775051FGP
CSB-EP775051FGP
CSB-BP775051FGP
CSB-MP775051FGP
CSB-EP775051FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Serine hydroxymethyltransferase 2 (glyA2) CSB-YP775052FGP
CSB-EP775052FGP
CSB-BP775052FGP
CSB-MP775052FGP
CSB-EP775052FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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