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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Serine/threonine-protein kinase PLK4 (plk4), partial CSB-YP773631DIL
CSB-EP773631DIL
CSB-BP773631DIL
CSB-MP773631DIL
CSB-EP773631DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Receptor expression-enhancing protein 3 (reep3), partial CSB-YP773632DIL1
CSB-EP773632DIL1
CSB-BP773632DIL1
CSB-MP773632DIL1
CSB-EP773632DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase NRDP1 (rnf41) CSB-YP773633DIL
CSB-EP773633DIL
CSB-BP773633DIL
CSB-MP773633DIL
CSB-EP773633DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Coiled-coil domain-containing protein 89 (ccdc89) CSB-YP773634XBE
CSB-EP773634XBE
CSB-BP773634XBE
CSB-MP773634XBE
CSB-EP773634XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Ubiquitin-like protein 4A (ubl4a) CSB-YP773635DIL
CSB-EP773635DIL
CSB-BP773635DIL
CSB-MP773635DIL
CSB-EP773635DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Proton-coupled folate transporter (slc46a1), partial CSB-YP773636DIL
CSB-EP773636DIL
CSB-BP773636DIL
CSB-MP773636DIL
CSB-EP773636DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Argininosuccinate synthase (ass1) CSB-YP773637XBE
CSB-EP773637XBE
CSB-BP773637XBE
CSB-MP773637XBE
CSB-EP773637XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cytosolic 5'-nucleotidase III-like protein A (nt5c3l-a) CSB-YP773638XBE
CSB-EP773638XBE
CSB-BP773638XBE
CSB-MP773638XBE
CSB-EP773638XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tetraspanin-31-B (tspan31-b), partial CSB-YP773639XBE1
CSB-EP773639XBE1
CSB-BP773639XBE1
CSB-MP773639XBE1
CSB-EP773639XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis WD repeat-containing protein 24 (wdr24), partial CSB-YP773640XBE
CSB-EP773640XBE
CSB-BP773640XBE
CSB-MP773640XBE
CSB-EP773640XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tyrosine--tRNA ligase, cytoplasmic (yars), partial CSB-YP773641XBE
CSB-EP773641XBE
CSB-BP773641XBE
CSB-MP773641XBE
CSB-EP773641XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis THO complex subunit 5 homolog B (thoc5-b), partial CSB-YP773642XBE
CSB-EP773642XBE
CSB-BP773642XBE
CSB-MP773642XBE
CSB-EP773642XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Testin (tes) CSB-YP773643XBE
CSB-EP773643XBE
CSB-BP773643XBE
CSB-MP773643XBE
CSB-EP773643XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mitogen-activated protein kinase kinase kinase 7-interacting protein 3 homolog (map3k7ip3), partial CSB-YP773644XBE
CSB-EP773644XBE
CSB-BP773644XBE
CSB-MP773644XBE
CSB-EP773644XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis CTP synthase 1-B (ctps-b), partial CSB-YP773645XBE
CSB-EP773645XBE
CSB-BP773645XBE
CSB-MP773645XBE
CSB-EP773645XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gap junction gamma-1 protein (gjc1), partial CSB-YP773646XBE1
CSB-EP773646XBE1
CSB-BP773646XBE1
CSB-MP773646XBE1
CSB-EP773646XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Kinesin-like protein KIF19 (kif19), partial CSB-YP773647XBE
CSB-EP773647XBE
CSB-BP773647XBE
CSB-MP773647XBE
CSB-EP773647XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ubiquitin-conjugating enzyme E2 T (ube2t) CSB-YP773648XBE
CSB-EP773648XBE
CSB-BP773648XBE
CSB-MP773648XBE
CSB-EP773648XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Acidic leucine-rich nuclear phosphoprotein 32 family member E (anp32e) CSB-YP773649XBE
CSB-EP773649XBE
CSB-BP773649XBE
CSB-MP773649XBE
CSB-EP773649XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Glucose-fructose oxidoreductase domain-containing protein 2 (gfod2) CSB-YP773650XBE
CSB-EP773650XBE
CSB-BP773650XBE
CSB-MP773650XBE
CSB-EP773650XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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