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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis UPF0406 protein C16orf57 homolog CSB-YP773851XBE
CSB-EP773851XBE
CSB-BP773851XBE
CSB-MP773851XBE
CSB-EP773851XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Myb/SANT-like DNA-binding domain-containing protein 3 (msantd3) CSB-YP773852XBE
CSB-EP773852XBE
CSB-BP773852XBE
CSB-MP773852XBE
CSB-EP773852XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Eukaryotic translation initiation factor 3 subunit M (eif3m) CSB-YP773853XBE
CSB-EP773853XBE
CSB-BP773853XBE
CSB-MP773853XBE
CSB-EP773853XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio ATPase family AAA domain-containing protein 1-A (atad1a) CSB-YP773854DIL
CSB-EP773854DIL
CSB-BP773854DIL
CSB-MP773854DIL
CSB-EP773854DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops jerdonii Cysteine-rich venom protein CSB-YP773855EZC
CSB-EP773855EZC
CSB-BP773855EZC
CSB-MP773855EZC
CSB-EP773855EZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DNA topoisomerase 2-binding protein 1-B (topbp1-B), partial CSB-YP773856XBE
CSB-EP773856XBE
CSB-BP773856XBE
CSB-MP773856XBE
CSB-EP773856XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Interferon-induced GTP-binding protein MxB (mxb), partial CSB-YP773857DIL
CSB-EP773857DIL
CSB-BP773857DIL
CSB-MP773857DIL
CSB-EP773857DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis CXXC-type zinc finger protein 4 (cxxc4) CSB-YP773858XBE
CSB-EP773858XBE
CSB-BP773858XBE
CSB-MP773858XBE
CSB-EP773858XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis LIM domain transcription factor LMO4-B (lmo4-b) CSB-YP773859XBE
CSB-EP773859XBE
CSB-BP773859XBE
CSB-MP773859XBE
CSB-EP773859XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 40S ribosomal protein S3a-A (rps3a-a) CSB-YP773860XBE
CSB-EP773860XBE
CSB-BP773860XBE
CSB-MP773860XBE
CSB-EP773860XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Squalus acanthias Serine/threonine-protein kinase 3/4 (STK4) CSB-YP773861FKW
CSB-EP773861FKW
CSB-BP773861FKW
CSB-MP773861FKW
CSB-EP773861FKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Structural maintenance of chromosomes protein 5 (smc5), partial CSB-YP773862TKY
CSB-EP773862TKY
CSB-BP773862TKY
CSB-MP773862TKY
CSB-EP773862TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein Jade-1 (phf17), partial CSB-YP773863DIL
CSB-EP773863DIL
CSB-BP773863DIL
CSB-MP773863DIL
CSB-EP773863DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Histone H3-like centromeric protein A (cenpa) CSB-YP773864DIL
CSB-EP773864DIL
CSB-BP773864DIL
CSB-MP773864DIL
CSB-EP773864DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein saal1 (saal1) CSB-YP773865DIL
CSB-EP773865DIL
CSB-BP773865DIL
CSB-MP773865DIL
CSB-EP773865DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Serine/threonine-protein phosphatase 2A regulatory subunit B' subunit gamma (ppp2r3c) CSB-YP773866DIL
CSB-EP773866DIL
CSB-BP773866DIL
CSB-MP773866DIL
CSB-EP773866DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Embryonic polyadenylate-binding protein 2-A (Pabpn1l-a) CSB-YP773867XBE
CSB-EP773867XBE
CSB-BP773867XBE
CSB-MP773867XBE
CSB-EP773867XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase mib1 (mib1), partial CSB-YP773868DIL
CSB-EP773868DIL
CSB-BP773868DIL
CSB-MP773868DIL
CSB-EP773868DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zinc finger protein 36, C3H1 type-like 2-B (zfp36l2-B) CSB-YP773869XBE
CSB-EP773869XBE
CSB-BP773869XBE
CSB-MP773869XBE
CSB-EP773869XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oreochromis mossambicus Brain natriuretic peptide (nppb) CSB-YP773870OES
CSB-EP773870OES
CSB-BP773870OES
CSB-MP773870OES
CSB-EP773870OES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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