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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Interferon-inducible double stranded RNA-dependent protein kinase activator A homolog A (prkra-a) CSB-YP773651XBE
CSB-EP773651XBE
CSB-BP773651XBE
CSB-MP773651XBE
CSB-EP773651XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Translationally-controlled tumor protein homolog (tpt1) CSB-YP773652XBE
CSB-EP773652XBE
CSB-BP773652XBE
CSB-MP773652XBE
CSB-EP773652XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Multivesicular body subunit 12A (fam125a) CSB-YP773653XBE
CSB-EP773653XBE
CSB-BP773653XBE
CSB-MP773653XBE
CSB-EP773653XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Forkhead box protein I1-ema (foxi1e) CSB-YP773654XBE
CSB-EP773654XBE
CSB-BP773654XBE
CSB-MP773654XBE
CSB-EP773654XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Disks large-associated protein 1 (dlgap1), partial CSB-YP773655DIL
CSB-EP773655DIL
CSB-BP773655DIL
CSB-MP773655DIL
CSB-EP773655DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Tetraspan membrane protein of hair cell stereocilia homolog (LHFPL5), partial CSB-YP773656CH1
CSB-EP773656CH1
CSB-BP773656CH1
CSB-MP773656CH1
CSB-EP773656CH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Nodal homolog 3-B (nodal3-B) CSB-YP773657XBF
CSB-EP773657XBF
CSB-BP773657XBF
CSB-MP773657XBF
CSB-EP773657XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes C-type natriuretic peptide 3 (cnp-3) CSB-YP773658OFH
CSB-EP773658OFH
CSB-BP773658OFH
CSB-MP773658OFH
CSB-EP773658OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Argyrosomus regius Osteocalcin (bglap) CSB-YP773659AAAH
CSB-EP773659AAAH
CSB-BP773659AAAH
CSB-MP773659AAAH
CSB-EP773659AAAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Doublesex- and mab-3-related transcription factor 1 (dmrt1) CSB-YP773660OFH
CSB-EP773660OFH
CSB-BP773660OFH
CSB-MP773660OFH
CSB-EP773660OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis AP-2 complex subunit mu (ap2m1) CSB-YP773661XBE
CSB-EP773661XBE
CSB-BP773661XBE
CSB-MP773661XBE
CSB-EP773661XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Methionine-R-sulfoxide reductase B1-A (msrb1) CSB-YP773662DIL
CSB-EP773662DIL
CSB-BP773662DIL
CSB-MP773662DIL
CSB-EP773662DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Aldehyde dehydrogenase family 9 member A1-B (aldh9a1b), partial CSB-YP773663DIL
CSB-EP773663DIL
CSB-BP773663DIL
CSB-MP773663DIL
CSB-EP773663DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Limb region 1 homolog-like protein (lmbr1l), partial CSB-YP773664DIL
CSB-EP773664DIL
CSB-BP773664DIL
CSB-MP773664DIL
CSB-EP773664DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0415 protein C7orf25 homolog (zgc:55781) CSB-YP773665DIL
CSB-EP773665DIL
CSB-BP773665DIL
CSB-MP773665DIL
CSB-EP773665DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein phosphatase 1 regulatory subunit 3B (ppp1r3b) CSB-YP773666DIL
CSB-EP773666DIL
CSB-BP773666DIL
CSB-MP773666DIL
CSB-EP773666DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Vacuolar fusion protein CCZ1 homolog (ccz1) CSB-YP773667DIL
CSB-EP773667DIL
CSB-BP773667DIL
CSB-MP773667DIL
CSB-EP773667DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Baculoviral IAP repeat-containing protein 5.2-B (birc5.2-b) CSB-YP773668XBE
CSB-EP773668XBE
CSB-BP773668XBE
CSB-MP773668XBE
CSB-EP773668XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 50S ribosomal protein L7/L12 (rplL) CSB-YP773688BTY
CSB-EP773688BTY
CSB-BP773688BTY
CSB-MP773688BTY
CSB-EP773688BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Thymidylate kinase (tmk) CSB-YP773689OBN
CSB-EP773689OBN
CSB-BP773689OBN
CSB-MP773689OBN
CSB-EP773689OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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