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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ajellomyces capsulatus Histone H2B (HTB1) CSB-YP773811AZC
CSB-EP773811AZC
CSB-BP773811AZC
CSB-MP773811AZC
CSB-EP773811AZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine recombinase XerC (xerC) CSB-YP773812VFI
CSB-EP773812VFI
CSB-BP773812VFI
CSB-MP773812VFI
CSB-EP773812VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine recombinase XerC (xerC) CSB-YP773813CQG
CSB-EP773813CQG
CSB-BP773813CQG
CSB-MP773813CQG
CSB-EP773813CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Segregation and condensation protein A (scpA) CSB-YP773814SMI
CSB-EP773814SMI
CSB-BP773814SMI
CSB-MP773814SMI
CSB-EP773814SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Segregation and condensation protein A (scpA) CSB-YP773815OAB
CSB-EP773815OAB
CSB-BP773815OAB
CSB-MP773815OAB
CSB-EP773815OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Tyrosine recombinase XerD (xerD) CSB-YP773816BGP
CSB-EP773816BGP
CSB-BP773816BGP
CSB-MP773816BGP
CSB-EP773816BGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RNA binding protein fox-1 homolog 1-like (rbfox1l) CSB-YP773817DIL
CSB-EP773817DIL
CSB-BP773817DIL
CSB-MP773817DIL
CSB-EP773817DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mitochondrial chaperone BCS1 (bcs1l), partial CSB-YP773818XBE1
CSB-EP773818XBE1
CSB-BP773818XBE1
CSB-MP773818XBE1
CSB-EP773818XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cairina moschata Follicle-stimulating hormone receptor (FSHR), partial CSB-YP773819CAG1
CSB-EP773819CAG1
CSB-BP773819CAG1
CSB-MP773819CAG1
CSB-EP773819CAG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica 40S ribosomal protein S17 (RPS17) CSB-YP773820DXJ
CSB-EP773820DXJ
CSB-BP773820DXJ
CSB-MP773820DXJ
CSB-EP773820DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UPF0568 protein C14orf166 homolog (zgc:56576) CSB-YP773821DIL
CSB-EP773821DIL
CSB-BP773821DIL
CSB-MP773821DIL
CSB-EP773821DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Serine/threonine-protein kinase VRK1 (vrk1) CSB-YP773822DIL
CSB-EP773822DIL
CSB-BP773822DIL
CSB-MP773822DIL
CSB-EP773822DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein Mdm4 (mdm4) CSB-YP773823DIL
CSB-EP773823DIL
CSB-BP773823DIL
CSB-MP773823DIL
CSB-EP773823DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RRP15-like protein (rrp15) CSB-YP773824DIL
CSB-EP773824DIL
CSB-BP773824DIL
CSB-MP773824DIL
CSB-EP773824DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Magnesium transporter protein 1 (magt1), partial CSB-YP773825DIL1
CSB-EP773825DIL1
CSB-BP773825DIL1
CSB-MP773825DIL1
CSB-EP773825DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Eukaryotic initiation factor 4A-III (eif4a3) CSB-YP773826DIL
CSB-EP773826DIL
CSB-BP773826DIL
CSB-MP773826DIL
CSB-EP773826DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio KDEL motif-containing protein 1 (kdelc1) CSB-YP773827DIL
CSB-EP773827DIL
CSB-BP773827DIL
CSB-MP773827DIL
CSB-EP773827DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Queuine tRNA-ribosyltransferase subunit qtrtd1 (qtrtd1) CSB-YP773828DIL
CSB-EP773828DIL
CSB-BP773828DIL
CSB-MP773828DIL
CSB-EP773828DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein Jade-3 (phf16), partial CSB-YP773829DIL
CSB-EP773829DIL
CSB-BP773829DIL
CSB-MP773829DIL
CSB-EP773829DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Spindle assembly abnormal protein 6 homolog (sass6), partial CSB-YP773830DIL
CSB-EP773830DIL
CSB-BP773830DIL
CSB-MP773830DIL
CSB-EP773830DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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