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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterococcus faecalis Redox-sensing transcriptional repressor rex 2 (rex2) CSB-YP774331ELW
CSB-EP774331ELW
CSB-BP774331ELW
CSB-MP774331ELW
CSB-EP774331ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice tungro spherical virus Genome polyprotein, partial CSB-YP774332RCAS1
CSB-EP774332RCAS1
CSB-BP774332RCAS1
CSB-MP774332RCAS1
CSB-EP774332RCAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lettuce infectious yellows virus Capsid protein CSB-YP774333LEY
CSB-EP774333LEY
CSB-BP774333LEY
CSB-MP774333LEY
CSB-EP774333LEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP774334ELW
CSB-EP774334ELW
CSB-BP774334ELW
CSB-MP774334ELW
CSB-EP774334ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Thiamine-phosphate synthase (thiE) CSB-YP774335ELW
CSB-EP774335ELW
CSB-BP774335ELW
CSB-MP774335ELW
CSB-EP774335ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Uncharacterized RNA methyltransferase EF_2706 (EF_2706) CSB-YP774336ELW
CSB-EP774336ELW
CSB-BP774336ELW
CSB-MP774336ELW
CSB-EP774336ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP774337ELW
CSB-EP774337ELW
CSB-BP774337ELW
CSB-MP774337ELW
CSB-EP774337ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis ATP synthase subunit a (atpB), partial CSB-YP774338ELW1
CSB-EP774338ELW1
CSB-BP774338ELW1
CSB-MP774338ELW1
CSB-EP774338ELW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis ATP synthase epsilon chain (atpC) CSB-YP774339ELW
CSB-EP774339ELW
CSB-BP774339ELW
CSB-MP774339ELW
CSB-EP774339ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Uracil phosphoribosyltransferase (upp) CSB-YP774340ELW
CSB-EP774340ELW
CSB-BP774340ELW
CSB-MP774340ELW
CSB-EP774340ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Phosphopantetheine adenylyltransferase (coaD) CSB-YP774341ELW
CSB-EP774341ELW
CSB-BP774341ELW
CSB-MP774341ELW
CSB-EP774341ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis GTPase Era (era) CSB-YP774342ELW
CSB-EP774342ELW
CSB-BP774342ELW
CSB-MP774342ELW
CSB-EP774342ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Asparagine--tRNA ligase (asnS) CSB-YP774343ELW
CSB-EP774343ELW
CSB-BP774343ELW
CSB-MP774343ELW
CSB-EP774343ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP774344CJAC
CSB-EP774344CJAC
CSB-BP774344CJAC
CSB-MP774344CJAC
CSB-EP774344CJAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus RNA-directed RNA polymerase 2a (ORF2a), partial CSB-YP774345CUE
CSB-EP774345CUE
CSB-BP774345CUE
CSB-MP774345CUE
CSB-EP774345CUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UPF0397 protein EF_2154 (EF_2154), partial CSB-YP774346ELW1
CSB-EP774346ELW1
CSB-BP774346ELW1
CSB-MP774346ELW1
CSB-EP774346ELW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Ferrochelatase (hemH) CSB-YP774347ELW
CSB-EP774347ELW
CSB-BP774347ELW
CSB-MP774347ELW
CSB-EP774347ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Probable GTP-binding protein EngB (engB) CSB-YP774348ELW
CSB-EP774348ELW
CSB-BP774348ELW
CSB-MP774348ELW
CSB-EP774348ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Aspartate 1-decarboxylase (panD) CSB-YP774349ELW
CSB-EP774349ELW
CSB-BP774349ELW
CSB-MP774349ELW
CSB-EP774349ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid protein VP4, partial CSB-YP774350RNY
CSB-EP774350RNY
CSB-BP774350RNY
CSB-MP774350RNY
CSB-EP774350RNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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