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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitrosomonas europaea 50S ribosomal protein L17 (rplQ) CSB-YP774311NHH
CSB-EP774311NHH
CSB-BP774311NHH
CSB-MP774311NHH
CSB-EP774311NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 30S ribosomal protein S14 (rpsN) CSB-YP774312NHH
CSB-EP774312NHH
CSB-BP774312NHH
CSB-MP774312NHH
CSB-EP774312NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 50S ribosomal protein L2 (rplB) CSB-YP774313NHH
CSB-EP774313NHH
CSB-BP774313NHH
CSB-MP774313NHH
CSB-EP774313NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ribonuclease P protein component (rnpA) CSB-YP774314NHH
CSB-EP774314NHH
CSB-BP774314NHH
CSB-MP774314NHH
CSB-EP774314NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) CSB-YP774315NHH
CSB-EP774315NHH
CSB-BP774315NHH
CSB-MP774315NHH
CSB-EP774315NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Flagellar P-ring protein (flgI) CSB-YP774316NHH
CSB-EP774316NHH
CSB-BP774316NHH
CSB-MP774316NHH
CSB-EP774316NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP774317NHH1
CSB-EP774317NHH1
CSB-BP774317NHH1
CSB-MP774317NHH1
CSB-EP774317NHH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea ATP synthase subunit alpha (atpA), partial CSB-YP774318NHH
CSB-EP774318NHH
CSB-BP774318NHH
CSB-MP774318NHH
CSB-EP774318NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Primosomal replication protein n (priB) CSB-YP774319NHH
CSB-EP774319NHH
CSB-BP774319NHH
CSB-MP774319NHH
CSB-EP774319NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP774320NHH
CSB-EP774320NHH
CSB-BP774320NHH
CSB-MP774320NHH
CSB-EP774320NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ribonuclease H (rnhA) CSB-YP774321NHH
CSB-EP774321NHH
CSB-BP774321NHH
CSB-MP774321NHH
CSB-EP774321NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP774322NHH
CSB-EP774322NHH
CSB-BP774322NHH
CSB-MP774322NHH
CSB-EP774322NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP774323NHH
CSB-EP774323NHH
CSB-BP774323NHH
CSB-MP774323NHH
CSB-EP774323NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP774324NHH
CSB-EP774324NHH
CSB-BP774324NHH
CSB-MP774324NHH
CSB-EP774324NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Chromosomal replication initiator protein DnaA (dnaA) CSB-YP774325NHH
CSB-EP774325NHH
CSB-BP774325NHH
CSB-MP774325NHH
CSB-EP774325NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP774326ELW
CSB-EP774326ELW
CSB-BP774326ELW
CSB-MP774326ELW
CSB-EP774326ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S9 (rpsI) CSB-YP774327ELW
CSB-EP774327ELW
CSB-BP774327ELW
CSB-MP774327ELW
CSB-EP774327ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Ribose-phosphate pyrophosphokinase 1 (prs1) CSB-YP774328ELW
CSB-EP774328ELW
CSB-BP774328ELW
CSB-MP774328ELW
CSB-EP774328ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Methionyl-tRNA formyltransferase (fmt) CSB-YP774329ELW
CSB-EP774329ELW
CSB-BP774329ELW
CSB-MP774329ELW
CSB-EP774329ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S15 (rpsO) CSB-YP774330ELW
CSB-EP774330ELW
CSB-BP774330ELW
CSB-MP774330ELW
CSB-EP774330ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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