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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterococcus faecalis Foldase protein prsA (prsA) CSB-YP774371ELW
CSB-EP774371ELW
CSB-BP774371ELW
CSB-MP774371ELW
CSB-EP774371ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant New York virus Envelope glycoprotein (GP), partial CSB-YP774372NCAE1
CSB-EP774372NCAE1
CSB-BP774372NCAE1
CSB-MP774372NCAE1
CSB-EP774372NCAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Superoxide dismutase [Fe] (sodA) CSB-YP774373ELW
CSB-EP774373ELW
CSB-BP774373ELW
CSB-MP774373ELW
CSB-EP774373ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Rhamnulokinase (rhaB) CSB-YP774374ELW
CSB-EP774374ELW
CSB-BP774374ELW
CSB-MP774374ELW
CSB-EP774374ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oat chlorotic stunt virus RNA-directed RNA polymerase (ORF1), partial CSB-YP774375OBAK
CSB-EP774375OBAK
CSB-BP774375OBAK
CSB-MP774375OBAK
CSB-EP774375OBAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus Uncharacterized 98.6 kDa protein (ORF71), partial CSB-YP774376OEU
CSB-EP774376OEU
CSB-BP774376OEU
CSB-MP774376OEU
CSB-EP774376OEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis tRNA (Ile)-lysidine synthase (tilS) CSB-YP774377ELW
CSB-EP774377ELW
CSB-BP774377ELW
CSB-MP774377ELW
CSB-EP774377ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP774378ELW
CSB-EP774378ELW
CSB-BP774378ELW
CSB-MP774378ELW
CSB-EP774378ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L18 (rplR) CSB-YP774379ELW
CSB-EP774379ELW
CSB-BP774379ELW
CSB-MP774379ELW
CSB-EP774379ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L14 (rplN) CSB-YP774380ELW
CSB-EP774380ELW
CSB-BP774380ELW
CSB-MP774380ELW
CSB-EP774380ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S19 (rpsS) CSB-YP774381ELW
CSB-EP774381ELW
CSB-BP774381ELW
CSB-MP774381ELW
CSB-EP774381ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S7 (rpsG) CSB-YP774382ELW
CSB-EP774382ELW
CSB-BP774382ELW
CSB-MP774382ELW
CSB-EP774382ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP774383ELW
CSB-EP774383ELW
CSB-BP774383ELW
CSB-MP774383ELW
CSB-EP774383ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Bifunctional protein GlmU (glmU) CSB-YP774384ELW
CSB-EP774384ELW
CSB-BP774384ELW
CSB-MP774384ELW
CSB-EP774384ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S18 (rpsR) CSB-YP774385ELW
CSB-EP774385ELW
CSB-BP774385ELW
CSB-MP774385ELW
CSB-EP774385ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Aspartate carbamoyltransferase (pyrB) CSB-YP774386DXP
CSB-EP774386DXP
CSB-BP774386DXP
CSB-MP774386DXP
CSB-EP774386DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-dehydroquinate dehydratase (aroD) CSB-YP774387DXP
CSB-EP774387DXP
CSB-BP774387DXP
CSB-MP774387DXP
CSB-EP774387DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Cystathionine beta-lyase (metC) CSB-YP774388DXP
CSB-EP774388DXP
CSB-BP774388DXP
CSB-MP774388DXP
CSB-EP774388DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Methionyl-tRNA formyltransferase (fmt) CSB-YP774389DXP
CSB-EP774389DXP
CSB-BP774389DXP
CSB-MP774389DXP
CSB-EP774389DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Release factor glutamine methyltransferase (prmC) CSB-YP774390DXP
CSB-EP774390DXP
CSB-BP774390DXP
CSB-MP774390DXP
CSB-EP774390DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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