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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida UPF0758 protein PP_5284 (PP_5284) CSB-YP775133FGB
CSB-EP775133FGB
CSB-BP775133FGB
CSB-MP775133FGB
CSB-EP775133FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Tyrosine recombinase XerC (xerC) CSB-YP775134FGB
CSB-EP775134FGB
CSB-BP775134FGB
CSB-MP775134FGB
CSB-EP775134FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Polyphosphate kinase (ppk), partial CSB-YP775135FGB
CSB-EP775135FGB
CSB-BP775135FGB
CSB-MP775135FGB
CSB-EP775135FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glycine dehydrogenase [decarboxylating] 2 (gcvP2), partial CSB-YP775136FGB
CSB-EP775136FGB
CSB-BP775136FGB
CSB-MP775136FGB
CSB-EP775136FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphopantetheine adenylyltransferase (coaD) CSB-YP775137FGB
CSB-EP775137FGB
CSB-BP775137FGB
CSB-MP775137FGB
CSB-EP775137FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA sulfurtransferase (thiI) CSB-YP775138FGB
CSB-EP775138FGB
CSB-BP775138FGB
CSB-MP775138FGB
CSB-EP775138FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glucans biosynthesis protein G (opgG), partial CSB-YP775139FGB
CSB-EP775139FGB
CSB-BP775139FGB
CSB-MP775139FGB
CSB-EP775139FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Putative Holliday junction resolvase (PP_4996) CSB-YP775140FGB
CSB-EP775140FGB
CSB-BP775140FGB
CSB-MP775140FGB
CSB-EP775140FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP775141FGB1
CSB-EP775141FGB1
CSB-BP775141FGB1
CSB-MP775141FGB1
CSB-EP775141FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA mismatch repair protein mutL (mutL), partial CSB-YP775142FGB
CSB-EP775142FGB
CSB-BP775142FGB
CSB-MP775142FGB
CSB-EP775142FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Adenylosuccinate synthetase (purA) CSB-YP775143FGB
CSB-EP775143FGB
CSB-BP775143FGB
CSB-MP775143FGB
CSB-EP775143FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP775144FGB
CSB-EP775144FGB
CSB-BP775144FGB
CSB-MP775144FGB
CSB-EP775144FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP775145FGB
CSB-EP775145FGB
CSB-BP775145FGB
CSB-MP775145FGB
CSB-EP775145FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP775146FGB
CSB-EP775146FGB
CSB-BP775146FGB
CSB-MP775146FGB
CSB-EP775146FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chaperone protein DnaJ (dnaJ) CSB-YP775147FGB
CSB-EP775147FGB
CSB-BP775147FGB
CSB-MP775147FGB
CSB-EP775147FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Triosephosphate isomerase (tpiA) CSB-YP775148FGB
CSB-EP775148FGB
CSB-BP775148FGB
CSB-MP775148FGB
CSB-EP775148FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glucose-6-phosphate isomerase 2 (pgi2), partial CSB-YP775149FGB
CSB-EP775149FGB
CSB-BP775149FGB
CSB-MP775149FGB
CSB-EP775149FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ketol-acid reductoisomerase (ilvC) CSB-YP775150FGB
CSB-EP775150FGB
CSB-BP775150FGB
CSB-MP775150FGB
CSB-EP775150FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Large-conductance mechanosensitive channel (mscL), partial CSB-YP775151FGB
CSB-EP775151FGB
CSB-BP775151FGB
CSB-MP775151FGB
CSB-EP775151FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP775152FGB
CSB-EP775152FGB
CSB-BP775152FGB
CSB-MP775152FGB
CSB-EP775152FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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