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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Apolipoprotein N-acyltransferase (lnt), partial CSB-YP801435VFE1
CSB-EP801435VFE1
CSB-BP801435VFE1
CSB-MP801435VFE1
CSB-EP801435VFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP801436VFE
CSB-EP801436VFE
CSB-BP801436VFE
CSB-MP801436VFE
CSB-EP801436VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N utilization substance protein B homolog (nusB) CSB-YP801437VFE
CSB-EP801437VFE
CSB-BP801437VFE
CSB-MP801437VFE
CSB-EP801437VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0255 protein VP0674 (VP0674) CSB-YP801438VFE
CSB-EP801438VFE
CSB-BP801438VFE
CSB-MP801438VFE
CSB-EP801438VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP801439VFE
CSB-EP801439VFE
CSB-BP801439VFE
CSB-MP801439VFE
CSB-EP801439VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP801440VFE
CSB-EP801440VFE
CSB-BP801440VFE
CSB-MP801440VFE
CSB-EP801440VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nucleoside diphosphate kinase (ndk) CSB-YP801441VFE
CSB-EP801441VFE
CSB-BP801441VFE
CSB-MP801441VFE
CSB-EP801441VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator iscR (iscR) CSB-YP801442VFE
CSB-EP801442VFE
CSB-BP801442VFE
CSB-MP801442VFE
CSB-EP801442VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein ClpB (clpB), partial CSB-YP801443VFE
CSB-EP801443VFE
CSB-BP801443VFE
CSB-MP801443VFE
CSB-EP801443VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate synthase (thyA) CSB-YP801444VFE
CSB-EP801444VFE
CSB-BP801444VFE
CSB-MP801444VFE
CSB-EP801444VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP801445VFE
CSB-EP801445VFE
CSB-BP801445VFE
CSB-MP801445VFE
CSB-EP801445VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0102 protein VP0448 (VP0448) CSB-YP801446VFE
CSB-EP801446VFE
CSB-BP801446VFE
CSB-MP801446VFE
CSB-EP801446VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP801447VFE
CSB-EP801447VFE
CSB-BP801447VFE
CSB-MP801447VFE
CSB-EP801447VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP801448VFE
CSB-EP801448VFE
CSB-BP801448VFE
CSB-MP801448VFE
CSB-EP801448VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP801449VFE
CSB-EP801449VFE
CSB-BP801449VFE
CSB-MP801449VFE
CSB-EP801449VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiamine import ATP-binding protein ThiQ (thiQ) CSB-YP801450VFE
CSB-EP801450VFE
CSB-BP801450VFE
CSB-MP801450VFE
CSB-EP801450VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP801451VFE
CSB-EP801451VFE
CSB-BP801451VFE
CSB-MP801451VFE
CSB-EP801451VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S14 (rpsN) CSB-YP801452VFE
CSB-EP801452VFE
CSB-BP801452VFE
CSB-MP801452VFE
CSB-EP801452VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16 (rplP) CSB-YP801453VFE
CSB-EP801453VFE
CSB-BP801453VFE
CSB-MP801453VFE
CSB-EP801453VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP801454VFE
CSB-EP801454VFE
CSB-BP801454VFE
CSB-MP801454VFE
CSB-EP801454VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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