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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant SsrA-binding protein (smpB) CSB-YP801708SJL
CSB-EP801708SJL
CSB-BP801708SJL
CSB-MP801708SJL
CSB-EP801708SJL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsular polysaccharide phosphotransferase xcbA (xcbA) CSB-YP801709NBJ
CSB-EP801709NBJ
CSB-BP801709NBJ
CSB-MP801709NBJ
CSB-EP801709NBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease P protein component (rnpA) CSB-YP801710TDAI
CSB-EP801710TDAI
CSB-BP801710TDAI
CSB-MP801710TDAI
CSB-EP801710TDAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative DNA ligase 4 (LIG4), partial CSB-YP801711OFG
CSB-EP801711OFG
CSB-BP801711OFG
CSB-MP801711OFG
CSB-EP801711OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Thioredoxin M2, chloroplastic (Os04g0530600, LOC_Os04g44830) CSB-YP801712OFG
CSB-EP801712OFG
CSB-BP801712OFG
CSB-MP801712OFG
CSB-EP801712OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (petC), partial CSB-YP801713TQN1
CSB-EP801713TQN1
CSB-BP801713TQN1
CSB-MP801713TQN1
CSB-EP801713TQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum bulbocastanum Putative disease resistance protein RGA1 (RGA1), partial CSB-YP801714STW
CSB-EP801714STW
CSB-BP801714STW
CSB-MP801714STW
CSB-EP801714STW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein TIFY 4A (TIFY4A) CSB-YP801715DOA
CSB-EP801715DOA
CSB-BP801715DOA
CSB-MP801715DOA
CSB-EP801715DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein DEHYDRATION-INDUCED 19 homolog 6 (DI19-6) CSB-YP801716OFG
CSB-EP801716OFG
CSB-BP801716OFG
CSB-MP801716OFG
CSB-EP801716OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Expansin-B6 (EXPB6) CSB-YP801717OFG
CSB-EP801717OFG
CSB-BP801717OFG
CSB-MP801717OFG
CSB-EP801717OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable cytokinin riboside 5'-monophosphate phosphoribohydrolase LOGL10 (LOGL10) CSB-YP801718OFG
CSB-EP801718OFG
CSB-BP801718OFG
CSB-MP801718OFG
CSB-EP801718OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative laccase-16 (LAC16) CSB-YP801719OFG
CSB-EP801719OFG
CSB-BP801719OFG
CSB-MP801719OFG
CSB-EP801719OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin transporter-like protein 3 (Os10g0147400, LOC_Os10g05690), partial CSB-YP801720OFG
CSB-EP801720OFG
CSB-BP801720OFG
CSB-MP801720OFG
CSB-EP801720OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 61 (Os07g0114000, LOC_Os07g02330) CSB-YP801721OFG
CSB-EP801721OFG
CSB-BP801721OFG
CSB-MP801721OFG
CSB-EP801721OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Homeobox-leucine zipper protein HOX14 (HOX14) CSB-YP801722OFG
CSB-EP801722OFG
CSB-BP801722OFG
CSB-MP801722OFG
CSB-EP801722OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum habrochaites Fructokinase-2 (FRK2) CSB-YP801723SZX
CSB-EP801723SZX
CSB-BP801723SZX
CSB-MP801723SZX
CSB-EP801723SZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamate receptor 3.1 (GLR3.1), partial CSB-YP801724DOA
CSB-EP801724DOA
CSB-BP801724DOA
CSB-MP801724DOA
CSB-EP801724DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein arginine N-methyltransferase 6.1 (PRMT6.1) CSB-YP801725OFG
CSB-EP801725OFG
CSB-BP801725OFG
CSB-MP801725OFG
CSB-EP801725OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable aquaporin TIP3-2 (TIP3-2), partial CSB-YP801726OFG1
CSB-EP801726OFG1
CSB-BP801726OFG1
CSB-MP801726OFG1
CSB-EP801726OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica B3 domain-containing protein Os04g0386900 (Os04g0386900, LOC_Os04g31730) CSB-YP801727OFG
CSB-EP801727OFG
CSB-BP801727OFG
CSB-MP801727OFG
CSB-EP801727OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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