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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas syringae pv. tomato tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP775073FGP
CSB-EP775073FGP
CSB-BP775073FGP
CSB-MP775073FGP
CSB-EP775073FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP775074FGP
CSB-EP775074FGP
CSB-BP775074FGP
CSB-MP775074FGP
CSB-EP775074FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Molybdenum import ATP-binding protein ModC (modC) CSB-YP775075FGP
CSB-EP775075FGP
CSB-BP775075FGP
CSB-MP775075FGP
CSB-EP775075FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP775076FGP
CSB-EP775076FGP
CSB-BP775076FGP
CSB-MP775076FGP
CSB-EP775076FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0502 protein PSPTO_2686 (PSPTO_2686) CSB-YP775077FGP
CSB-EP775077FGP
CSB-BP775077FGP
CSB-MP775077FGP
CSB-EP775077FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphonates import ATP-binding protein PhnC 2 (phnC2) CSB-YP775078FGP
CSB-EP775078FGP
CSB-BP775078FGP
CSB-MP775078FGP
CSB-EP775078FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato D-ribose pyranase (rbsD) CSB-YP775079FGP
CSB-EP775079FGP
CSB-BP775079FGP
CSB-MP775079FGP
CSB-EP775079FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Transcription elongation factor GreB (greB) CSB-YP775080FGP
CSB-EP775080FGP
CSB-BP775080FGP
CSB-MP775080FGP
CSB-EP775080FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Chaperone protein htpG (htpG), partial CSB-YP775081FGP
CSB-EP775081FGP
CSB-BP775081FGP
CSB-MP775081FGP
CSB-EP775081FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-C74 Uncharacterized protein ORF14 (ORF14) CSB-YP775082SGAH
CSB-EP775082SGAH
CSB-BP775082SGAH
CSB-MP775082SGAH
CSB-EP775082SGAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sendai virus Matrix protein (M) CSB-YP775083SEW
CSB-EP775083SEW
CSB-BP775083SEW
CSB-MP775083SEW
CSB-EP775083SEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UvrABC system protein B (uvrB), partial CSB-YP775084FGP
CSB-EP775084FGP
CSB-BP775084FGP
CSB-MP775084FGP
CSB-EP775084FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP775085FGP
CSB-EP775085FGP
CSB-BP775085FGP
CSB-MP775085FGP
CSB-EP775085FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Chorismate synthase (aroC) CSB-YP775086FGP
CSB-EP775086FGP
CSB-BP775086FGP
CSB-MP775086FGP
CSB-EP775086FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon (cheB1) CSB-YP775087FGP
CSB-EP775087FGP
CSB-BP775087FGP
CSB-MP775087FGP
CSB-EP775087FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 6,7-dimethyl-8-ribityllumazine synthase 2 (ribH2) CSB-YP775088FGP
CSB-EP775088FGP
CSB-BP775088FGP
CSB-MP775088FGP
CSB-EP775088FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP775089FGP
CSB-EP775089FGP
CSB-BP775089FGP
CSB-MP775089FGP
CSB-EP775089FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphoserine aminotransferase (serC) CSB-YP775090FGP
CSB-EP775090FGP
CSB-BP775090FGP
CSB-MP775090FGP
CSB-EP775090FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Cobyric acid synthase (cobQ) CSB-YP775091FGP
CSB-EP775091FGP
CSB-BP775091FGP
CSB-MP775091FGP
CSB-EP775091FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP775092FGP
CSB-EP775092FGP
CSB-BP775092FGP
CSB-MP775092FGP
CSB-EP775092FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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