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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas syringae pv. tomato tRNA pseudouridine synthase D (truD) CSB-YP775093FGP
CSB-EP775093FGP
CSB-BP775093FGP
CSB-MP775093FGP
CSB-EP775093FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP775094FGP
CSB-EP775094FGP
CSB-BP775094FGP
CSB-MP775094FGP
CSB-EP775094FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Lysine--tRNA ligase (lysS) CSB-YP775095FGP
CSB-EP775095FGP
CSB-BP775095FGP
CSB-MP775095FGP
CSB-EP775095FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 50S ribosomal protein L19 (rplS) CSB-YP775096FGP
CSB-EP775096FGP
CSB-BP775096FGP
CSB-MP775096FGP
CSB-EP775096FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP775097FGP
CSB-EP775097FGP
CSB-BP775097FGP
CSB-MP775097FGP
CSB-EP775097FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Nucleoside diphosphate kinase (ndk) CSB-YP775098FGP
CSB-EP775098FGP
CSB-BP775098FGP
CSB-MP775098FGP
CSB-EP775098FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Type III secretion ATP synthase HrcN (hrcN) CSB-YP775099FGP
CSB-EP775099FGP
CSB-BP775099FGP
CSB-MP775099FGP
CSB-EP775099FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Disulfide bond formation protein B (dsbB), partial CSB-YP775100FGP1
CSB-EP775100FGP1
CSB-BP775100FGP1
CSB-MP775100FGP1
CSB-EP775100FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Alginate biosynthesis protein AlgK (algK) CSB-YP775101FGP
CSB-EP775101FGP
CSB-BP775101FGP
CSB-MP775101FGP
CSB-EP775101FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Alginate biosynthesis protein AlgA (algA) CSB-YP775102FGP
CSB-EP775102FGP
CSB-BP775102FGP
CSB-MP775102FGP
CSB-EP775102FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco ringspot virus Capsid protein, partial CSB-YP775103TCAK
CSB-EP775103TCAK
CSB-BP775103TCAK
CSB-MP775103TCAK
CSB-EP775103TCAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glutamate racemase (murI) CSB-YP775104FGP
CSB-EP775104FGP
CSB-BP775104FGP
CSB-MP775104FGP
CSB-EP775104FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable sugar efflux transporter (sotB), partial CSB-YP775105FGP
CSB-EP775105FGP
CSB-BP775105FGP
CSB-MP775105FGP
CSB-EP775105FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP775106FGP
CSB-EP775106FGP
CSB-BP775106FGP
CSB-MP775106FGP
CSB-EP775106FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Effector protein hopD1 (hopD1), partial CSB-YP775107FGP
CSB-EP775107FGP
CSB-BP775107FGP
CSB-MP775107FGP
CSB-EP775107FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Walleye dermal sarcoma virus Envelope glycoprotein (env), partial CSB-YP775108WBH1
CSB-EP775108WBH1
CSB-BP775108WBH1
CSB-MP775108WBH1
CSB-EP775108WBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Isoleucine--tRNA ligase (ileS), partial CSB-YP775109FGP
CSB-EP775109FGP
CSB-BP775109FGP
CSB-MP775109FGP
CSB-EP775109FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato tRNA 2-selenouridine synthase (selU) CSB-YP775110FGP
CSB-EP775110FGP
CSB-BP775110FGP
CSB-MP775110FGP
CSB-EP775110FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP775111FGP
CSB-EP775111FGP
CSB-BP775111FGP
CSB-MP775111FGP
CSB-EP775111FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Transcriptional repressor NrdR (nrdR) CSB-YP775112FGP
CSB-EP775112FGP
CSB-BP775112FGP
CSB-MP775112FGP
CSB-EP775112FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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