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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum 26S proteasome non-ATPase regulatory subunit 14 (psmD14) CSB-YP801194DKK
CSB-EP801194DKK
CSB-BP801194DKK
CSB-MP801194DKK
CSB-EP801194DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Calcium-binding protein K (cbpK) CSB-YP801195DKK
CSB-EP801195DKK
CSB-BP801195DKK
CSB-MP801195DKK
CSB-EP801195DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Dolichyldiphosphatase 1 (dolpp1), partial CSB-YP801196DKK1
CSB-EP801196DKK1
CSB-BP801196DKK1
CSB-MP801196DKK1
CSB-EP801196DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ubiquitin-conjugating enzyme E2 pex4 (pex4) CSB-YP801197DKK
CSB-EP801197DKK
CSB-BP801197DKK
CSB-MP801197DKK
CSB-EP801197DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ragulator complex protein LAMTOR3 homolog (DDB_G0274833) CSB-YP801198DKK
CSB-EP801198DKK
CSB-BP801198DKK
CSB-MP801198DKK
CSB-EP801198DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Recoverin family protein DDB_G0272130 (DDB_G0272130) CSB-YP801199DKK
CSB-EP801199DKK
CSB-BP801199DKK
CSB-MP801199DKK
CSB-EP801199DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cln5-like protein 1 (cln5la), partial CSB-YP801200DKK1
CSB-EP801200DKK1
CSB-BP801200DKK1
CSB-MP801200DKK1
CSB-EP801200DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative elongation of fatty acids protein DDB_G0272012 (DDB_G0272012), partial CSB-YP801201DKK1
CSB-EP801201DKK1
CSB-BP801201DKK1
CSB-MP801201DKK1
CSB-EP801201DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum 60S ribosomal protein L15 (rpl15-1) CSB-YP801202DKK
CSB-EP801202DKK
CSB-BP801202DKK
CSB-MP801202DKK
CSB-EP801202DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable 28S ribosomal protein S17, mitochondrial (mrps17), partial CSB-YP801203DKK
CSB-EP801203DKK
CSB-BP801203DKK
CSB-MP801203DKK
CSB-EP801203DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0274405 (DDB_G0274405) CSB-YP801204DKK
CSB-EP801204DKK
CSB-BP801204DKK
CSB-MP801204DKK
CSB-EP801204DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cyclin-C (cycC) CSB-YP801205DKK
CSB-EP801205DKK
CSB-BP801205DKK
CSB-MP801205DKK
CSB-EP801205DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Bis (5'-adenosyl)-triphosphatase (fhit) CSB-YP801206DKK
CSB-EP801206DKK
CSB-BP801206DKK
CSB-MP801206DKK
CSB-EP801206DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Prolyl 4-hydroxylase subunit alpha (phyA) CSB-YP801207DKK
CSB-EP801207DKK
CSB-BP801207DKK
CSB-MP801207DKK
CSB-EP801207DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum TNF receptor-associated factor family protein DDB_G0272348 (DDB_G0272348), partial CSB-YP801208DKK
CSB-EP801208DKK
CSB-BP801208DKK
CSB-MP801208DKK
CSB-EP801208DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Rho GTPase-activating protein gacHH (gacHH), partial CSB-YP801209DKK
CSB-EP801209DKK
CSB-BP801209DKK
CSB-MP801209DKK
CSB-EP801209DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-hexosaminidase subunit beta (HEXB), partial CSB-YP801210EKM
CSB-EP801210EKM
CSB-BP801210EKM
CSB-MP801210EKM
CSB-EP801210EKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Negative elongation factor A (Nelf-A), partial CSB-YP801211DLU
CSB-EP801211DLU
CSB-BP801211DLU
CSB-MP801211DLU
CSB-EP801211DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostrea edulis Sperm-specific H1/protamine-like protein type 2 CSB-YP801212OBAG
CSB-EP801212OBAG
CSB-BP801212OBAG
CSB-MP801212OBAG
CSB-EP801212OBAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides sculpturatus Potassium channel toxin gamma-KTx 5.1 (ERG5) CSB-YP801213CGD
CSB-EP801213CGD
CSB-BP801213CGD
CSB-MP801213CGD
CSB-EP801213CGD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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