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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Penicillium chrysogenum Autophagy protein 5 (atg5) CSB-YP002293ETH
CSB-EP002293ETH
CSB-BP002293ETH
CSB-MP002293ETH
CSB-EP002293ETH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia franciscana 40S ribosomal protein S3a CSB-YP020444DOF
CSB-EP020444DOF
CSB-BP020444DOF
CSB-MP020444DOF
CSB-EP020444DOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Reticulon-1 (rtn1), partial CSB-YP020568XBF1
CSB-EP020568XBF1
CSB-BP020568XBF1
CSB-MP020568XBF1
CSB-EP020568XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Cysteine desulfurase (iscS), partial CSB-YP015769CXL
CSB-EP015769CXL
CSB-BP015769CXL
CSB-MP015769CXL
CSB-EP015769CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii tRNA pseudouridine synthase A (truA) CSB-YP019086CXL
CSB-EP019086CXL
CSB-BP019086CXL
CSB-MP019086CXL
CSB-EP019086CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Beta-galactosidase (lacZ), partial CSB-YP009476CXL
CSB-EP009476CXL
CSB-BP009476CXL
CSB-MP009476CXL
CSB-EP009476CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Translation initiation factor IF-2 (infB), partial CSB-YP015164CXL
CSB-EP015164CXL
CSB-BP015164CXL
CSB-MP015164CXL
CSB-EP015164CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi tRNA pseudouridine synthase A (truA) CSB-YP019086VEA
CSB-EP019086VEA
CSB-BP019086VEA
CSB-MP019086VEA
CSB-EP019086VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Lipoyl synthase (lipA) CSB-YP012927VEA
CSB-EP012927VEA
CSB-BP012927VEA
CSB-MP012927VEA
CSB-EP012927VEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Elongation factor G (fusA), partial CSB-YP009373FDV
CSB-EP009373FDV
CSB-BP009373FDV
CSB-MP009373FDV
CSB-EP009373FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ferrochelatase (hemH) CSB-YP008579FDV
CSB-EP008579FDV
CSB-BP008579FDV
CSB-MP008579FDV
CSB-EP008579FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA dimethylallyltransferase (miaA) CSB-YP024529FDV
CSB-EP024529FDV
CSB-BP024529FDV
CSB-MP024529FDV
CSB-EP024529FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Malate dehydrogenase (mdh) CSB-YP013623FDV
CSB-EP013623FDV
CSB-BP013623FDV
CSB-MP013623FDV
CSB-EP013623FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica tRNA pseudouridine synthase A (truA) CSB-YP019086FDV
CSB-EP019086FDV
CSB-BP019086FDV
CSB-MP019086FDV
CSB-EP019086FDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Nucleoside diphosphate kinase (ndk) CSB-YP015889RIP
CSB-EP015889RIP
CSB-BP015889RIP
CSB-MP015889RIP
CSB-EP015889RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Malate dehydrogenase (mdh) CSB-YP013623RIP
CSB-EP013623RIP
CSB-BP013623RIP
CSB-MP013623RIP
CSB-EP013623RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917RIP
CSB-EP022917RIP
CSB-BP022917RIP
CSB-MP022917RIP
CSB-EP022917RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Roseiflexus castenholzii Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824RIP
CSB-EP005824RIP
CSB-BP005824RIP
CSB-MP005824RIP
CSB-EP005824RIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis Methylthioribose-1-phosphate isomerase (v1g237765) CSB-YP014799NGO
CSB-EP014799NGO
CSB-BP014799NGO
CSB-MP014799NGO
CSB-EP014799NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nematostella vectensis tRNA-splicing ligase RtcB homolog (v1g198406), partial CSB-YP003810NGO
CSB-EP003810NGO
CSB-BP003810NGO
CSB-MP003810NGO
CSB-EP003810NGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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