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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vanderwaltozyma polyspora Nucleolar protein 58 (NOP58), partial CSB-YP015940VDW
CSB-EP015940VDW
CSB-BP015940VDW
CSB-MP015940VDW
CSB-EP015940VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora U6 snRNA-associated Sm-like protein LSm6 (LSM6) CSB-YP013209VDW
CSB-EP013209VDW
CSB-BP013209VDW
CSB-MP013209VDW
CSB-EP013209VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora V-type proton ATPase subunit F (VMA7) CSB-YP002407VDW
CSB-EP002407VDW
CSB-BP002407VDW
CSB-MP002407VDW
CSB-EP002407VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Thymidylate kinase (tmk) CSB-YP007226YAN
CSB-EP007226YAN
CSB-BP007226YAN
CSB-MP007226YAN
CSB-EP007226YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Phosphoserine aminotransferase (serC) CSB-YP018838YAN
CSB-EP018838YAN
CSB-BP018838YAN
CSB-MP018838YAN
CSB-EP018838YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917YAN
CSB-EP022917YAN
CSB-BP022917YAN
CSB-MP022917YAN
CSB-EP022917YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b tRNA dimethylallyltransferase (miaA) CSB-YP024529YAN
CSB-EP024529YAN
CSB-BP024529YAN
CSB-MP024529YAN
CSB-EP024529YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Peptide deformylase (def) CSB-YP017707YAN
CSB-EP017707YAN
CSB-BP017707YAN
CSB-MP017707YAN
CSB-EP017707YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b ATP synthase subunit b (atpF), partial CSB-YP002358YAN1
CSB-EP002358YAN1
CSB-BP002358YAN1
CSB-MP002358YAN1
CSB-EP002358YAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Serine hydroxymethyltransferase (glyA) CSB-YP021274CWU
CSB-EP021274CWU
CSB-BP021274CWU
CSB-MP021274CWU
CSB-EP021274CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTPase Era (era) CSB-YP007759CWU
CSB-EP007759CWU
CSB-BP007759CWU
CSB-MP007759CWU
CSB-EP007759CWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033CWW
CSB-EP018033CWW
CSB-BP018033CWW
CSB-MP018033CWW
CSB-EP018033CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase subunit alpha (atpA), partial CSB-YP002344CWW
CSB-EP002344CWW
CSB-BP002344CWW
CSB-MP002344CWW
CSB-EP002344CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Argininosuccinate synthase (argG) CSB-YP002234CWW
CSB-EP002234CWW
CSB-BP002234CWW
CSB-MP002234CWW
CSB-EP002234CWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis Thymidylate synthase (thyA) CSB-YP025393BOK
CSB-EP025393BOK
CSB-BP025393BOK
CSB-MP025393BOK
CSB-EP025393BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus subsp. cytotoxis S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658BOK
CSB-EP001658BOK
CSB-BP001658BOK
CSB-MP001658BOK
CSB-EP001658BOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Translation initiation factor IF-3 (infC) CSB-YP015166CWB
CSB-EP015166CWB
CSB-BP015166CWB
CSB-MP015166CWB
CSB-EP015166CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus tRNA dimethylallyltransferase (miaA) CSB-YP024529CWB
CSB-EP024529CWB
CSB-BP024529CWB
CSB-MP024529CWB
CSB-EP024529CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Ribonuclease 3 (rnc) CSB-YP019809CWB
CSB-EP019809CWB
CSB-BP019809CWB
CSB-MP019809CWB
CSB-EP019809CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Thymidylate kinase (tmk) CSB-YP007226CWE
CSB-EP007226CWE
CSB-BP007226CWE
CSB-MP007226CWE
CSB-EP007226CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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