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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Demethylmenaquinone methyltransferase (ubiE) CSB-YP800531BQE
CSB-EP800531BQE
CSB-BP800531BQE
CSB-MP800531BQE
CSB-EP800531BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavohemoprotein (hmp) CSB-YP800532BQE
CSB-EP800532BQE
CSB-BP800532BQE
CSB-MP800532BQE
CSB-EP800532BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP800533BQE
CSB-EP800533BQE
CSB-BP800533BQE
CSB-MP800533BQE
CSB-EP800533BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ketol-acid reductoisomerase 1 (ilvC1) CSB-YP800534BQE
CSB-EP800534BQE
CSB-BP800534BQE
CSB-MP800534BQE
CSB-EP800534BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--poly (phosphoribitol) ligase subunit 2 (dltC) CSB-YP800535BQE
CSB-EP800535BQE
CSB-BP800535BQE
CSB-MP800535BQE
CSB-EP800535BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP800536BQE
CSB-EP800536BQE
CSB-BP800536BQE
CSB-MP800536BQE
CSB-EP800536BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate phosphoribosyltransferase (trpD) CSB-YP800537BQE
CSB-EP800537BQE
CSB-BP800537BQE
CSB-MP800537BQE
CSB-EP800537BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0413 protein BA_1208/GBAA_1208/BAS1115 (BA_1208, GBAA_1208, BAS1115) CSB-YP800538BQE
CSB-EP800538BQE
CSB-BP800538BQE
CSB-MP800538BQE
CSB-EP800538BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0344 protein BA_1155/GBAA_1155/BAS1072 (BA_1155, GBAA_1155, BAS1072), partial CSB-YP800539BQE1
CSB-EP800539BQE1
CSB-BP800539BQE1
CSB-MP800539BQE1
CSB-EP800539BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferrochelatase 1 (hemH1) CSB-YP800540BQE
CSB-EP800540BQE
CSB-BP800540BQE
CSB-MP800540BQE
CSB-EP800540BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0145 protein BA_1001/GBAA_1001/BAS0936 (BA_1001, GBAA_1001, BAS0936) CSB-YP800541BQE
CSB-EP800541BQE
CSB-BP800541BQE
CSB-MP800541BQE
CSB-EP800541BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable disulfide formation protein C 1 (bdbC1), partial CSB-YP800542BQE1
CSB-EP800542BQE1
CSB-BP800542BQE1
CSB-MP800542BQE1
CSB-EP800542BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-ribose pyranase (rbsD) CSB-YP800543BQE
CSB-EP800543BQE
CSB-BP800543BQE
CSB-MP800543BQE
CSB-EP800543BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP800544BQE
CSB-EP800544BQE
CSB-BP800544BQE
CSB-MP800544BQE
CSB-EP800544BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine import ATP-binding protein MetN 1 (metN1) CSB-YP800545BQE
CSB-EP800545BQE
CSB-BP800545BQE
CSB-MP800545BQE
CSB-EP800545BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP800546BQE
CSB-EP800546BQE
CSB-BP800546BQE
CSB-MP800546BQE
CSB-EP800546BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L30 (rpmD) CSB-YP800547BQE
CSB-EP800547BQE
CSB-BP800547BQE
CSB-MP800547BQE
CSB-EP800547BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L5 (rplE) CSB-YP800548BQE
CSB-EP800548BQE
CSB-BP800548BQE
CSB-MP800548BQE
CSB-EP800548BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L22 (rplV) CSB-YP800549BQE
CSB-EP800549BQE
CSB-BP800549BQE
CSB-MP800549BQE
CSB-EP800549BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fusA), partial CSB-YP800550BQE
CSB-EP800550BQE
CSB-BP800550BQE
CSB-MP800550BQE
CSB-EP800550BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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