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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L1 (rplA) CSB-YP800551BQE
CSB-EP800551BQE
CSB-BP800551BQE
CSB-MP800551BQE
CSB-EP800551BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA integrity scanning protein DisA (disA) CSB-YP800552BQE
CSB-EP800552BQE
CSB-BP800552BQE
CSB-MP800552BQE
CSB-EP800552BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein GlmU (glmU) CSB-YP800553BQE
CSB-EP800553BQE
CSB-BP800553BQE
CSB-MP800553BQE
CSB-EP800553BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0213 protein BA_0032/GBAA_0032/BAS0034 (BA_0032, GBAA_0032, BAS0034) CSB-YP800554BQE
CSB-EP800554BQE
CSB-BP800554BQE
CSB-MP800554BQE
CSB-EP800554BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP800555BQE
CSB-EP800555BQE
CSB-BP800555BQE
CSB-MP800555BQE
CSB-EP800555BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized N-acetyltransferase BA_4060/GBAA_4060/BAS3772 (BA_4060, GBAA_4060, BAS3772) CSB-YP800556BQE
CSB-EP800556BQE
CSB-BP800556BQE
CSB-MP800556BQE
CSB-EP800556BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP800557BQE
CSB-EP800557BQE
CSB-BP800557BQE
CSB-MP800557BQE
CSB-EP800557BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase (pyrB) CSB-YP800558BQE
CSB-EP800558BQE
CSB-BP800558BQE
CSB-MP800558BQE
CSB-EP800558BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orotate phosphoribosyltransferase (pyrE) CSB-YP800559BQE
CSB-EP800559BQE
CSB-BP800559BQE
CSB-MP800559BQE
CSB-EP800559BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine/threonine-protein kinase PrkC (prkC), partial CSB-YP800560BQE
CSB-EP800560BQE
CSB-BP800560BQE
CSB-MP800560BQE
CSB-EP800560BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease 3 (rnc) CSB-YP800561BQE
CSB-EP800561BQE
CSB-BP800561BQE
CSB-MP800561BQE
CSB-EP800561BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent protease subunit HslV (hslV) CSB-YP800562BQE
CSB-EP800562BQE
CSB-BP800562BQE
CSB-MP800562BQE
CSB-EP800562BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-recycling factor (frr) CSB-YP800563BQE
CSB-EP800563BQE
CSB-BP800563BQE
CSB-MP800563BQE
CSB-EP800563BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP800564BQE
CSB-EP800564BQE
CSB-BP800564BQE
CSB-MP800564BQE
CSB-EP800564BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA mismatch repair protein MutS (mutS), partial CSB-YP800565BQE
CSB-EP800565BQE
CSB-BP800565BQE
CSB-MP800565BQE
CSB-EP800565BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spermidine synthase 2 (speE2) CSB-YP800566BQE
CSB-EP800566BQE
CSB-BP800566BQE
CSB-MP800566BQE
CSB-EP800566BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative sporulation transcription regulator WhiA (whiA) CSB-YP800567BQE
CSB-EP800567BQE
CSB-BP800567BQE
CSB-MP800567BQE
CSB-EP800567BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP800568BQE
CSB-EP800568BQE
CSB-BP800568BQE
CSB-MP800568BQE
CSB-EP800568BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine--tRNA ligase 2 (tyrS2) CSB-YP800569BQE
CSB-EP800569BQE
CSB-BP800569BQE
CSB-MP800569BQE
CSB-EP800569BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine import ATP-binding protein MetN 3 (metN3) CSB-YP800570BQE
CSB-EP800570BQE
CSB-BP800570BQE
CSB-MP800570BQE
CSB-EP800570BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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