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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitrosomonas europaea 5'-nucleotidase surE (surE) CSB-YP800731NHH
CSB-EP800731NHH
CSB-BP800731NHH
CSB-MP800731NHH
CSB-EP800731NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Histidinol dehydrogenase (hisD) CSB-YP800732NHH
CSB-EP800732NHH
CSB-BP800732NHH
CSB-MP800732NHH
CSB-EP800732NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP800733NHH
CSB-EP800733NHH
CSB-BP800733NHH
CSB-MP800733NHH
CSB-EP800733NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Dihydroorotase (pyrC) CSB-YP800734NHH
CSB-EP800734NHH
CSB-BP800734NHH
CSB-MP800734NHH
CSB-EP800734NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP800735NHH
CSB-EP800735NHH
CSB-BP800735NHH
CSB-MP800735NHH
CSB-EP800735NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP800736NHH
CSB-EP800736NHH
CSB-BP800736NHH
CSB-MP800736NHH
CSB-EP800736NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Dihydrodipicolinate reductase (dapB) CSB-YP800737NHH
CSB-EP800737NHH
CSB-BP800737NHH
CSB-MP800737NHH
CSB-EP800737NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP800738NHH
CSB-EP800738NHH
CSB-BP800738NHH
CSB-MP800738NHH
CSB-EP800738NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP800739NHH
CSB-EP800739NHH
CSB-BP800739NHH
CSB-MP800739NHH
CSB-EP800739NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 30S ribosomal protein S5 (rpsE) CSB-YP800740NHH
CSB-EP800740NHH
CSB-BP800740NHH
CSB-MP800740NHH
CSB-EP800740NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 30S ribosomal protein S19 (rpsS) CSB-YP800741NHH
CSB-EP800741NHH
CSB-BP800741NHH
CSB-MP800741NHH
CSB-EP800741NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea UPF0235 protein NE0395 (NE0395) CSB-YP800742NHH
CSB-EP800742NHH
CSB-BP800742NHH
CSB-MP800742NHH
CSB-EP800742NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Maf-like protein NE0356 (NE0356) CSB-YP800743NHH
CSB-EP800743NHH
CSB-BP800743NHH
CSB-MP800743NHH
CSB-EP800743NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Thiazole synthase (thiG) CSB-YP800744NHH
CSB-EP800744NHH
CSB-BP800744NHH
CSB-MP800744NHH
CSB-EP800744NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 7-cyano-7-deazaguanine synthase (queC) CSB-YP800745NHH
CSB-EP800745NHH
CSB-BP800745NHH
CSB-MP800745NHH
CSB-EP800745NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea ATP synthase subunit b (atpF), partial CSB-YP800746NHH
CSB-EP800746NHH
CSB-BP800746NHH
CSB-MP800746NHH
CSB-EP800746NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 30S ribosomal protein S18 (rpsR) CSB-YP800747NHH
CSB-EP800747NHH
CSB-BP800747NHH
CSB-MP800747NHH
CSB-EP800747NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP800748NHH
CSB-EP800748NHH
CSB-BP800748NHH
CSB-MP800748NHH
CSB-EP800748NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable CRISPR-associated endoribonuclease Cas2 1 (cas21) CSB-YP800749NHH
CSB-EP800749NHH
CSB-BP800749NHH
CSB-MP800749NHH
CSB-EP800749NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Oligoribonuclease (orn) CSB-YP800750NHH
CSB-EP800750NHH
CSB-BP800750NHH
CSB-MP800750NHH
CSB-EP800750NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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