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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant UPF0229 protein BA_0551/GBAA_0551/BAS0519 (BA_0551, GBAA_0551, BAS0519) CSB-YP802454BQE
CSB-EP802454BQE
CSB-BP802454BQE
CSB-MP802454BQE
CSB-EP802454BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutaminase 1 (glsA1) CSB-YP802455BQE
CSB-EP802455BQE
CSB-BP802455BQE
CSB-MP802455BQE
CSB-EP802455BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA topoisomerase 3 (topB), partial CSB-YP802456BQE
CSB-EP802456BQE
CSB-BP802456BQE
CSB-MP802456BQE
CSB-EP802456BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP802457BQE
CSB-EP802457BQE
CSB-BP802457BQE
CSB-MP802457BQE
CSB-EP802457BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP802458BQE
CSB-EP802458BQE
CSB-BP802458BQE
CSB-MP802458BQE
CSB-EP802458BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP802459NHH
CSB-EP802459NHH
CSB-BP802459NHH
CSB-MP802459NHH
CSB-EP802459NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 8-amino-7-oxononanoate synthase (bioF) CSB-YP802460NHH
CSB-EP802460NHH
CSB-BP802460NHH
CSB-MP802460NHH
CSB-EP802460NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP802461RGD
CSB-EP802461RGD
CSB-BP802461RGD
CSB-MP802461RGD
CSB-EP802461RGD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hirame rhabdovirus Matrix protein (M) CSB-YP802462HDAL
CSB-EP802462HDAL
CSB-BP802462HDAL
CSB-MP802462HDAL
CSB-EP802462HDAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 3 CSB-YP802463RGQ
CSB-EP802463RGQ
CSB-BP802463RGQ
CSB-MP802463RGQ
CSB-EP802463RGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP802464NHH
CSB-EP802464NHH
CSB-BP802464NHH
CSB-MP802464NHH
CSB-EP802464NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apolipoprotein N-acyltransferase (lnt), partial CSB-YP802465SZB1
CSB-EP802465SZB1
CSB-BP802465SZB1
CSB-MP802465SZB1
CSB-EP802465SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP802466TIV
CSB-EP802466TIV
CSB-BP802466TIV
CSB-MP802466TIV
CSB-EP802466TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP802467DSL
CSB-EP802467DSL
CSB-BP802467DSL
CSB-MP802467DSL
CSB-EP802467DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP802468SNN
CSB-EP802468SNN
CSB-BP802468SNN
CSB-MP802468SNN
CSB-EP802468SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP802469NHH
CSB-EP802469NHH
CSB-BP802469NHH
CSB-MP802469NHH
CSB-EP802469NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 2-isopropylmalate synthase (leuA), partial CSB-YP802470NHH
CSB-EP802470NHH
CSB-BP802470NHH
CSB-MP802470NHH
CSB-EP802470NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea tRNA pseudouridine synthase A (truA) CSB-YP802471NHH
CSB-EP802471NHH
CSB-BP802471NHH
CSB-MP802471NHH
CSB-EP802471NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 50S ribosomal protein L24 (rplX) CSB-YP802472NHH
CSB-EP802472NHH
CSB-BP802472NHH
CSB-MP802472NHH
CSB-EP802472NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP802473ELW
CSB-EP802473ELW
CSB-BP802473ELW
CSB-MP802473ELW
CSB-EP802473ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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