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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Acylphosphatase (yccX) CSB-YP800931SZB
CSB-EP800931SZB
CSB-BP800931SZB
CSB-MP800931SZB
CSB-EP800931SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP800932SZB
CSB-EP800932SZB
CSB-BP800932SZB
CSB-MP800932SZB
CSB-EP800932SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LPS-assembly lipoprotein lptE (lptE) CSB-YP800933SZB
CSB-EP800933SZB
CSB-BP800933SZB
CSB-MP800933SZB
CSB-EP800933SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galactokinase (galK) CSB-YP800934SZB
CSB-EP800934SZB
CSB-BP800934SZB
CSB-MP800934SZB
CSB-EP800934SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl esterase (aes) CSB-YP800935SZB
CSB-EP800935SZB
CSB-BP800935SZB
CSB-MP800935SZB
CSB-EP800935SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Recombination-associated protein RdgC (rdgC) CSB-YP800936SZB
CSB-EP800936SZB
CSB-BP800936SZB
CSB-MP800936SZB
CSB-EP800936SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant [Protein-PII] uridylyltransferase (glnD), partial CSB-YP800937SZB
CSB-EP800937SZB
CSB-BP800937SZB
CSB-MP800937SZB
CSB-EP800937SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulokinase (araB), partial CSB-YP800938SZB
CSB-EP800938SZB
CSB-BP800938SZB
CSB-MP800938SZB
CSB-EP800938SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP800939SZB
CSB-EP800939SZB
CSB-BP800939SZB
CSB-MP800939SZB
CSB-EP800939SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endonuclease V (nfi) CSB-YP800940SZB
CSB-EP800940SZB
CSB-BP800940SZB
CSB-MP800940SZB
CSB-EP800940SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD-dependent malic enzyme (maeA), partial CSB-YP800941SZB
CSB-EP800941SZB
CSB-BP800941SZB
CSB-MP800941SZB
CSB-EP800941SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Na (+)/H (+) antiporter nhaA (nhaA), partial CSB-YP800942TIW
CSB-EP800942TIW
CSB-BP800942TIW
CSB-MP800942TIW
CSB-EP800942TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Thymidylate synthase thyX (thyX) CSB-YP800943TIW
CSB-EP800943TIW
CSB-BP800943TIW
CSB-MP800943TIW
CSB-EP800943TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei GTPase Era (era) CSB-YP800944TIW
CSB-EP800944TIW
CSB-BP800944TIW
CSB-MP800944TIW
CSB-EP800944TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP800945TIW
CSB-EP800945TIW
CSB-BP800945TIW
CSB-MP800945TIW
CSB-EP800945TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Membrane protein insertase YidC (yidC), partial CSB-YP800946TIV1
CSB-EP800946TIV1
CSB-BP800946TIV1
CSB-MP800946TIV1
CSB-EP800946TIV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP800947TIV
CSB-EP800947TIV
CSB-BP800947TIV
CSB-MP800947TIV
CSB-EP800947TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Acyl carrier protein (acpP) CSB-YP800948TIV
CSB-EP800948TIV
CSB-BP800948TIV
CSB-MP800948TIV
CSB-EP800948TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Sec-independent protein translocase protein TatA (tatA), partial CSB-YP800949TIV
CSB-EP800949TIV
CSB-BP800949TIV
CSB-MP800949TIV
CSB-EP800949TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Elongation factor Ts (tsf) CSB-YP800950TIV
CSB-EP800950TIV
CSB-BP800950TIV
CSB-MP800950TIV
CSB-EP800950TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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