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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nitrosomonas europaea Phosphomethylpyrimidine synthase (thiC), partial CSB-YP800751NHH
CSB-EP800751NHH
CSB-BP800751NHH
CSB-MP800751NHH
CSB-EP800751NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Anthranilate phosphoribosyltransferase (trpD) CSB-YP800752NHH
CSB-EP800752NHH
CSB-BP800752NHH
CSB-MP800752NHH
CSB-EP800752NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP800753ELW
CSB-EP800753ELW
CSB-BP800753ELW
CSB-MP800753ELW
CSB-EP800753ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Sensor protein lytS (lytS), partial CSB-YP800754ELW1
CSB-EP800754ELW1
CSB-BP800754ELW1
CSB-MP800754ELW1
CSB-EP800754ELW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Guanylate kinase (gmk) CSB-YP800755ELW
CSB-EP800755ELW
CSB-BP800755ELW
CSB-MP800755ELW
CSB-EP800755ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Ribonuclease 3 (rnc) CSB-YP800756ELW
CSB-EP800756ELW
CSB-BP800756ELW
CSB-MP800756ELW
CSB-EP800756ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Serine/threonine transporter sstT (sstT), partial CSB-YP800757ELW
CSB-EP800757ELW
CSB-BP800757ELW
CSB-MP800757ELW
CSB-EP800757ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UPF0356 protein EF_2923 (EF_2923) CSB-YP800758ELW
CSB-EP800758ELW
CSB-BP800758ELW
CSB-MP800758ELW
CSB-EP800758ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lettuce infectious yellows virus Protein p26 CSB-YP800759LEY
CSB-EP800759LEY
CSB-BP800759LEY
CSB-MP800759LEY
CSB-EP800759LEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Probable tautomerase EF_2859 (EF_2859) CSB-YP800760ELW
CSB-EP800760ELW
CSB-BP800760ELW
CSB-MP800760ELW
CSB-EP800760ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Thymidylate kinase (tmk) CSB-YP800761ELW
CSB-EP800761ELW
CSB-BP800761ELW
CSB-MP800761ELW
CSB-EP800761ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L1 (rplA) CSB-YP800762ELW
CSB-EP800762ELW
CSB-BP800762ELW
CSB-MP800762ELW
CSB-EP800762ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Tryptophan--tRNA ligase (trpS) CSB-YP800763ELW
CSB-EP800763ELW
CSB-BP800763ELW
CSB-MP800763ELW
CSB-EP800763ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Magnesium transporter mgtE (mgtE), partial CSB-YP800764ELW1
CSB-EP800764ELW1
CSB-BP800764ELW1
CSB-MP800764ELW1
CSB-EP800764ELW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis ATP synthase subunit alpha (atpA), partial CSB-YP800765ELW
CSB-EP800765ELW
CSB-BP800765ELW
CSB-MP800765ELW
CSB-EP800765ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Thymidine kinase (tdk) CSB-YP800766ELW
CSB-EP800766ELW
CSB-BP800766ELW
CSB-MP800766ELW
CSB-EP800766ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Teichoic acids export ATP-binding protein TagH (tagH) CSB-YP800767ELW
CSB-EP800767ELW
CSB-BP800767ELW
CSB-MP800767ELW
CSB-EP800767ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Undecaprenyl-diphosphatase (uppP), partial CSB-YP800768ELW1
CSB-EP800768ELW1
CSB-BP800768ELW1
CSB-MP800768ELW1
CSB-EP800768ELW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Probable rRNA maturation factor (EF_2412) CSB-YP800769ELW
CSB-EP800769ELW
CSB-BP800769ELW
CSB-MP800769ELW
CSB-EP800769ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Elongation factor Ts (tsf) CSB-YP800770ELW
CSB-EP800770ELW
CSB-BP800770ELW
CSB-MP800770ELW
CSB-EP800770ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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