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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Tropheryma whipplei Chromosomal replication initiator protein DnaA (dnaA) CSB-YP800951TIV
CSB-EP800951TIV
CSB-BP800951TIV
CSB-MP800951TIV
CSB-EP800951TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (ulaD) CSB-YP800952SZB
CSB-EP800952SZB
CSB-BP800952SZB
CSB-MP800952SZB
CSB-EP800952SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Divalent-cation tolerance protein CutA (cutA) CSB-YP800953SZB
CSB-EP800953SZB
CSB-BP800953SZB
CSB-MP800953SZB
CSB-EP800953SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multidrug resistance protein MdtN (mdtN), partial CSB-YP800954SZB
CSB-EP800954SZB
CSB-BP800954SZB
CSB-MP800954SZB
CSB-EP800954SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphomethylpyrimidine synthase (thiC), partial CSB-YP800955SZB
CSB-EP800955SZB
CSB-BP800955SZB
CSB-MP800955SZB
CSB-EP800955SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP800956SZB
CSB-EP800956SZB
CSB-BP800956SZB
CSB-MP800956SZB
CSB-EP800956SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein FdhD (fdhD) CSB-YP800957SZB
CSB-EP800957SZB
CSB-BP800957SZB
CSB-MP800957SZB
CSB-EP800957SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-ketoacyl-CoA thiolase (fadA) CSB-YP800958SZB
CSB-EP800958SZB
CSB-BP800958SZB
CSB-MP800958SZB
CSB-EP800958SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-ribose pyranase (rbsD) CSB-YP800959SZB
CSB-EP800959SZB
CSB-BP800959SZB
CSB-MP800959SZB
CSB-EP800959SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit delta (atpH) CSB-YP800960SZB
CSB-EP800960SZB
CSB-BP800960SZB
CSB-MP800960SZB
CSB-EP800960SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease PH (rph) CSB-YP800961SZB
CSB-EP800961SZB
CSB-BP800961SZB
CSB-MP800961SZB
CSB-EP800961SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycogen synthase (glgA) CSB-YP800962SZB
CSB-EP800962SZB
CSB-BP800962SZB
CSB-MP800962SZB
CSB-EP800962SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator frlR (frlR) CSB-YP800963SZB
CSB-EP800963SZB
CSB-BP800963SZB
CSB-MP800963SZB
CSB-EP800963SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP800964SZB
CSB-EP800964SZB
CSB-BP800964SZB
CSB-MP800964SZB
CSB-EP800964SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP800965SZB
CSB-EP800965SZB
CSB-BP800965SZB
CSB-MP800965SZB
CSB-EP800965SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S9 (rpsI) CSB-YP800966SZB
CSB-EP800966SZB
CSB-BP800966SZB
CSB-MP800966SZB
CSB-EP800966SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L (+)-tartrate dehydratase subunit alpha (ttdA) CSB-YP800967SZB
CSB-EP800967SZB
CSB-BP800967SZB
CSB-MP800967SZB
CSB-EP800967SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-fucose mutarotase (fucU) CSB-YP800968SZB
CSB-EP800968SZB
CSB-BP800968SZB
CSB-MP800968SZB
CSB-EP800968SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP800969SZB
CSB-EP800969SZB
CSB-BP800969SZB
CSB-MP800969SZB
CSB-EP800969SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein hscA homolog (hscA), partial CSB-YP800970SZB
CSB-EP800970SZB
CSB-BP800970SZB
CSB-MP800970SZB
CSB-EP800970SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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