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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pinus koraiensis Photosystem I reaction center subunit VIII (psaI), partial CSB-YP801114PYR
CSB-EP801114PYR
CSB-BP801114PYR
CSB-MP801114PYR
CSB-EP801114PYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus koraiensis Photosystem I reaction center subunit IX (psaJ), partial CSB-YP801115PYR
CSB-EP801115PYR
CSB-BP801115PYR
CSB-MP801115PYR
CSB-EP801115PYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus koraiensis Maturase K (matK), partial CSB-YP801116PYR
CSB-EP801116PYR
CSB-BP801116PYR
CSB-MP801116PYR
CSB-EP801116PYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus koraiensis Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP801117PYR
CSB-EP801117PYR
CSB-BP801117PYR
CSB-MP801117PYR
CSB-EP801117PYR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psathyrostachys juncea Maturase K (matK), partial CSB-YP801118PGAZ
CSB-EP801118PGAZ
CSB-BP801118PGAZ
CSB-MP801118PGAZ
CSB-EP801118PGAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice gall dwarf virus Outer capsid protein P3, partial CSB-YP801119RMJ
CSB-EP801119RMJ
CSB-BP801119RMJ
CSB-MP801119RMJ
CSB-EP801119RMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes NF-kappa-B inhibitor-like protein 1 (NFKBIL1) CSB-YP801120EQV
CSB-EP801120EQV
CSB-BP801120EQV
CSB-MP801120EQV
CSB-EP801120EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colobus guereza Angiogenin (ANG) CSB-YP801121DVH
CSB-EP801121DVH
CSB-BP801121DVH
CSB-MP801121DVH
CSB-EP801121DVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Intermediate capsid protein VP6 CSB-YP801122RCAU
CSB-EP801122RCAU
CSB-BP801122RCAU
CSB-MP801122RCAU
CSB-EP801122RCAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diadromus pulchellus idnoreovirus 1 Uncharacterized protein S2 (S2), partial CSB-YP801123DCAU
CSB-EP801123DCAU
CSB-BP801123DCAU
CSB-MP801123DCAU
CSB-EP801123DCAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human respiratory syncytial virus A Non-structural protein 2 (1B) CSB-YP801124HPP
CSB-EP801124HPP
CSB-BP801124HPP
CSB-MP801124HPP
CSB-EP801124HPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Trefoil factor 3 (TFF3) CSB-BP801125DO
CSB-EP801125DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Axis porcinus Sex-determining region Y protein (SRY) CSB-YP801126ATQ
CSB-EP801126ATQ
CSB-BP801126ATQ
CSB-MP801126ATQ
CSB-EP801126ATQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tarsius syrichta Cytochrome c oxidase subunit 8B, mitochondrial (COX8B), partial CSB-YP801127TLJ
CSB-EP801127TLJ
CSB-BP801127TLJ
CSB-MP801127TLJ
CSB-EP801127TLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ateles belzebuth Cytochrome c oxidase subunit 8A, mitochondrial (COX8A), partial CSB-YP801128AQG
CSB-EP801128AQG
CSB-BP801128AQG
CSB-MP801128AQG
CSB-EP801128AQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Prokineticin-2 (PROK2) CSB-YP801129BO
CSB-EP801129BO
CSB-BP801129BO
CSB-MP801129BO
CSB-EP801129BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca assamensis Ribonuclease-like protein 9 (RNASE9) CSB-YP801130MMV
CSB-EP801130MMV
CSB-BP801130MMV
CSB-MP801130MMV
CSB-EP801130MMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Solute carrier family 22 member 1 (SLC22A1), partial CSB-YP801131PI
CSB-EP801131PI
CSB-BP801131PI
CSB-MP801131PI
CSB-EP801131PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lemur catta Melanocyte-stimulating hormone receptor (MC1R), partial CSB-YP801132LOK1
CSB-EP801132LOK1
CSB-BP801132LOK1
CSB-MP801132LOK1
CSB-EP801132LOK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loris tardigradus Melanocyte-stimulating hormone receptor (MC1R), partial CSB-YP801133LLW1
CSB-EP801133LLW1
CSB-BP801133LLW1
CSB-MP801133LLW1
CSB-EP801133LLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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