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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa 50S ribosomal protein L14 (rplN) CSB-YP803235XAT
CSB-EP803235XAT
CSB-BP803235XAT
CSB-MP803235XAT
CSB-EP803235XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L22 (rplV) CSB-YP803236XAT
CSB-EP803236XAT
CSB-BP803236XAT
CSB-MP803236XAT
CSB-EP803236XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L3 (rplC) CSB-YP803237XAT
CSB-EP803237XAT
CSB-BP803237XAT
CSB-MP803237XAT
CSB-EP803237XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0307 protein PD_0419 (PD_0419) CSB-YP803238XAT
CSB-EP803238XAT
CSB-BP803238XAT
CSB-MP803238XAT
CSB-EP803238XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Carbamoyl-phosphate synthase small chain (carA) CSB-YP803239XAT
CSB-EP803239XAT
CSB-BP803239XAT
CSB-MP803239XAT
CSB-EP803239XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0350 protein PD_0354 (PD_0354) CSB-YP803240XAT
CSB-EP803240XAT
CSB-BP803240XAT
CSB-MP803240XAT
CSB-EP803240XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Putative zinc metalloprotease PD_0327 (PD_0327), partial CSB-YP803241XAT1
CSB-EP803241XAT1
CSB-BP803241XAT1
CSB-MP803241XAT1
CSB-EP803241XAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP803242XAT
CSB-EP803242XAT
CSB-BP803242XAT
CSB-MP803242XAT
CSB-EP803242XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Putative membrane protein insertion efficiency factor (PD_0282) CSB-YP803243XAT
CSB-EP803243XAT
CSB-BP803243XAT
CSB-MP803243XAT
CSB-EP803243XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP803244XAT
CSB-EP803244XAT
CSB-BP803244XAT
CSB-MP803244XAT
CSB-EP803244XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Triosephosphate isomerase (tpiA) CSB-YP803245XAT
CSB-EP803245XAT
CSB-BP803245XAT
CSB-MP803245XAT
CSB-EP803245XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Pantothenate synthetase (panC) CSB-YP803246XAT
CSB-EP803246XAT
CSB-BP803246XAT
CSB-MP803246XAT
CSB-EP803246XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP803247XAT
CSB-EP803247XAT
CSB-BP803247XAT
CSB-MP803247XAT
CSB-EP803247XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Valine--tRNA ligase (valS), partial CSB-YP803248XAT
CSB-EP803248XAT
CSB-BP803248XAT
CSB-MP803248XAT
CSB-EP803248XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S16 (rpsP) CSB-YP803249XAT
CSB-EP803249XAT
CSB-BP803249XAT
CSB-MP803249XAT
CSB-EP803249XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP803250XAT
CSB-EP803250XAT
CSB-BP803250XAT
CSB-MP803250XAT
CSB-EP803250XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP803251XAT
CSB-EP803251XAT
CSB-BP803251XAT
CSB-MP803251XAT
CSB-EP803251XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uronate isomerase (uxaC) CSB-YP803252VFE
CSB-EP803252VFE
CSB-BP803252VFE
CSB-MP803252VFE
CSB-EP803252VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabinose import ATP-binding protein AraG (araG), partial CSB-YP803253VFE
CSB-EP803253VFE
CSB-BP803253VFE
CSB-MP803253VFE
CSB-EP803253VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0176 protein VPA1597 (VPA1597) CSB-YP803254VFE
CSB-EP803254VFE
CSB-BP803254VFE
CSB-MP803254VFE
CSB-EP803254VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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