Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitrosomonas europaea Putative pterin-4-alpha-carbinolamine dehydratase (NE0077) CSB-YP802634NHH
CSB-EP802634NHH
CSB-BP802634NHH
CSB-MP802634NHH
CSB-EP802634NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Undecaprenyl-diphosphatase (uppP), partial CSB-YP802635NHH1
CSB-EP802635NHH1
CSB-BP802635NHH1
CSB-MP802635NHH1
CSB-EP802635NHH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L34 (rpmH) CSB-YP802636ELW
CSB-EP802636ELW
CSB-BP802636ELW
CSB-MP802636ELW
CSB-EP802636ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Serine--tRNA ligase 2 (serS2) CSB-YP802637ELW
CSB-EP802637ELW
CSB-BP802637ELW
CSB-MP802637ELW
CSB-EP802637ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Putative competence-damage inducible protein (cinA) CSB-YP802638ELW
CSB-EP802638ELW
CSB-BP802638ELW
CSB-MP802638ELW
CSB-EP802638ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Mannonate dehydratase (uxuA) CSB-YP802639ELW
CSB-EP802639ELW
CSB-BP802639ELW
CSB-MP802639ELW
CSB-EP802639ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Glutathione biosynthesis bifunctional protein gshAB (gshAB), partial CSB-YP802640ELW
CSB-EP802640ELW
CSB-BP802640ELW
CSB-MP802640ELW
CSB-EP802640ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Putative ribose uptake protein rbsU (rbsU), partial CSB-YP802641ELW
CSB-EP802641ELW
CSB-BP802641ELW
CSB-MP802641ELW
CSB-EP802641ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Ferredoxin--NADP reductase (EF_2899) CSB-YP802642ELW
CSB-EP802642ELW
CSB-BP802642ELW
CSB-MP802642ELW
CSB-EP802642ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lettuce infectious yellows virus Replicase polyprotein 1ab, partial CSB-YP802643LEY
CSB-EP802643LEY
CSB-BP802643LEY
CSB-MP802643LEY
CSB-EP802643LEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP802644ELW
CSB-EP802644ELW
CSB-BP802644ELW
CSB-MP802644ELW
CSB-EP802644ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UPF0133 protein EF_2780 (EF_2780) CSB-YP802645ELW
CSB-EP802645ELW
CSB-BP802645ELW
CSB-MP802645ELW
CSB-EP802645ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP802646ELW
CSB-EP802646ELW
CSB-BP802646ELW
CSB-MP802646ELW
CSB-EP802646ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Regulatory protein recX (recX) CSB-YP802647ELW
CSB-EP802647ELW
CSB-BP802647ELW
CSB-MP802647ELW
CSB-EP802647ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP802648ELW
CSB-EP802648ELW
CSB-BP802648ELW
CSB-MP802648ELW
CSB-EP802648ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis ATP synthase subunit delta (atpH) CSB-YP802649ELW
CSB-EP802649ELW
CSB-BP802649ELW
CSB-MP802649ELW
CSB-EP802649ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Peptide chain release factor 1 (prfA) CSB-YP802650ELW
CSB-EP802650ELW
CSB-BP802650ELW
CSB-MP802650ELW
CSB-EP802650ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP802651ELW
CSB-EP802651ELW
CSB-BP802651ELW
CSB-MP802651ELW
CSB-EP802651ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S20 (rpsT) CSB-YP802652ELW
CSB-EP802652ELW
CSB-BP802652ELW
CSB-MP802652ELW
CSB-EP802652ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Putative phosphotransferase EF_2419 (EF_2419) CSB-YP802653ELW
CSB-EP802653ELW
CSB-BP802653ELW
CSB-MP802653ELW
CSB-EP802653ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>