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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Pseudomonas syringae pv. tomato UPF0313 protein PSPTO_4928 (PSPTO_4928), partial CSB-YP801475FGP
CSB-EP801475FGP
CSB-BP801475FGP
CSB-MP801475FGP
CSB-EP801475FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Urease accessory protein UreD 2 (ureD2) CSB-YP801476FGP
CSB-EP801476FGP
CSB-BP801476FGP
CSB-MP801476FGP
CSB-EP801476FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Gamma-glutamyl phosphate reductase (proA) CSB-YP801477FGP
CSB-EP801477FGP
CSB-BP801477FGP
CSB-MP801477FGP
CSB-EP801477FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Leucine--tRNA ligase (leuS), partial CSB-YP801478FGP
CSB-EP801478FGP
CSB-BP801478FGP
CSB-MP801478FGP
CSB-EP801478FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP801479FGP
CSB-EP801479FGP
CSB-BP801479FGP
CSB-MP801479FGP
CSB-EP801479FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP801480FGP
CSB-EP801480FGP
CSB-BP801480FGP
CSB-MP801480FGP
CSB-EP801480FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Peptide chain release factor 3 (prfC), partial CSB-YP801481FGP
CSB-EP801481FGP
CSB-BP801481FGP
CSB-MP801481FGP
CSB-EP801481FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP801482FGP
CSB-EP801482FGP
CSB-BP801482FGP
CSB-MP801482FGP
CSB-EP801482FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Ribosome-binding factor A (rbfA) CSB-YP801483FGP
CSB-EP801483FGP
CSB-BP801483FGP
CSB-MP801483FGP
CSB-EP801483FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0307 protein PSPTO_4464 (PSPTO_4464) CSB-YP801484FGP
CSB-EP801484FGP
CSB-BP801484FGP
CSB-MP801484FGP
CSB-EP801484FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP801485FGP
CSB-EP801485FGP
CSB-BP801485FGP
CSB-MP801485FGP
CSB-EP801485FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP801486FGP
CSB-EP801486FGP
CSB-BP801486FGP
CSB-MP801486FGP
CSB-EP801486FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0234 protein PSPTO_4393 (PSPTO_4393) CSB-YP801487FGP
CSB-EP801487FGP
CSB-BP801487FGP
CSB-MP801487FGP
CSB-EP801487FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato GTPase Era (era) CSB-YP801488FGP
CSB-EP801488FGP
CSB-BP801488FGP
CSB-MP801488FGP
CSB-EP801488FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glycerol kinase (glpK), partial CSB-YP801489FGP
CSB-EP801489FGP
CSB-BP801489FGP
CSB-MP801489FGP
CSB-EP801489FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Deoxycytidine triphosphate deaminase (dcd) CSB-YP801490FGP
CSB-EP801490FGP
CSB-BP801490FGP
CSB-MP801490FGP
CSB-EP801490FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato RNA polymerase-associated protein RapA (rapA), partial CSB-YP801491FGP
CSB-EP801491FGP
CSB-BP801491FGP
CSB-MP801491FGP
CSB-EP801491FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Aspartate--tRNA ligase (aspS), partial CSB-YP801492FGP
CSB-EP801492FGP
CSB-BP801492FGP
CSB-MP801492FGP
CSB-EP801492FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Quinolinate synthase A (nadA) CSB-YP801493FGP
CSB-EP801493FGP
CSB-BP801493FGP
CSB-MP801493FGP
CSB-EP801493FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Cell division topological specificity factor (minE) CSB-YP801494FGP
CSB-EP801494FGP
CSB-BP801494FGP
CSB-MP801494FGP
CSB-EP801494FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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