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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida 50S ribosomal protein L4 (rplD) CSB-YP801635FGB
CSB-EP801635FGB
CSB-BP801635FGB
CSB-MP801635FGB
CSB-EP801635FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S12 (rpsL) CSB-YP801636FGB
CSB-EP801636FGB
CSB-BP801636FGB
CSB-MP801636FGB
CSB-EP801636FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP801637FGB
CSB-EP801637FGB
CSB-BP801637FGB
CSB-MP801637FGB
CSB-EP801637FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP801638FGB
CSB-EP801638FGB
CSB-BP801638FGB
CSB-MP801638FGB
CSB-EP801638FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Coenzyme PQQ synthesis protein F (pqqF), partial CSB-YP801639FGB
CSB-EP801639FGB
CSB-BP801639FGB
CSB-MP801639FGB
CSB-EP801639FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP801640FGB
CSB-EP801640FGB
CSB-BP801640FGB
CSB-MP801640FGB
CSB-EP801640FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Agmatine deiminase (aguA) CSB-YP801641FGB
CSB-EP801641FGB
CSB-BP801641FGB
CSB-MP801641FGB
CSB-EP801641FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP801642FGB
CSB-EP801642FGB
CSB-BP801642FGB
CSB-MP801642FGB
CSB-EP801642FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Zinc import ATP-binding protein ZnuC (znuC) CSB-YP801643FGB
CSB-EP801643FGB
CSB-BP801643FGB
CSB-MP801643FGB
CSB-EP801643FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP801644FGB
CSB-EP801644FGB
CSB-BP801644FGB
CSB-MP801644FGB
CSB-EP801644FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Membrane protein insertase YidC 2 (yidC2), partial CSB-YP801645LMS1
CSB-EP801645LMS1
CSB-BP801645LMS1
CSB-MP801645LMS1
CSB-EP801645LMS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Inosose dehydratase (iolE) CSB-YP801646LMS
CSB-EP801646LMS
CSB-BP801646LMS
CSB-MP801646LMS
CSB-EP801646LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Probable phosphoketolase 2 (lp_3551), partial CSB-YP801647LMS
CSB-EP801647LMS
CSB-BP801647LMS
CSB-MP801647LMS
CSB-EP801647LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Demethylmenaquinone methyltransferase (ubiE) CSB-YP801648LMS
CSB-EP801648LMS
CSB-BP801648LMS
CSB-MP801648LMS
CSB-EP801648LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP801649LMS
CSB-EP801649LMS
CSB-BP801649LMS
CSB-MP801649LMS
CSB-EP801649LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP801650LMS
CSB-EP801650LMS
CSB-BP801650LMS
CSB-MP801650LMS
CSB-EP801650LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Putative sugar uptake protein lp_2594 (lp_2594), partial CSB-YP801651LMS
CSB-EP801651LMS
CSB-BP801651LMS
CSB-MP801651LMS
CSB-EP801651LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP801652LMS
CSB-EP801652LMS
CSB-BP801652LMS
CSB-MP801652LMS
CSB-EP801652LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum UPF0210 protein lp_2507 (lp_2507) CSB-YP801653LMS
CSB-EP801653LMS
CSB-BP801653LMS
CSB-MP801653LMS
CSB-EP801653LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Serine hydroxymethyltransferase (glyA) CSB-YP801654LMS
CSB-EP801654LMS
CSB-BP801654LMS
CSB-MP801654LMS
CSB-EP801654LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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