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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida UPF0246 protein PP_1289 (PP_1289) CSB-YP801615FGB
CSB-EP801615FGB
CSB-BP801615FGB
CSB-MP801615FGB
CSB-EP801615FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Alginate production protein AlgE (algE) CSB-YP801616FGB
CSB-EP801616FGB
CSB-BP801616FGB
CSB-MP801616FGB
CSB-EP801616FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Alginate biosynthesis protein AlgF (algF) CSB-YP801617FGB
CSB-EP801617FGB
CSB-BP801617FGB
CSB-MP801617FGB
CSB-EP801617FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP801618FGB
CSB-EP801618FGB
CSB-BP801618FGB
CSB-MP801618FGB
CSB-EP801618FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable potassium transport system protein kup (kup), partial CSB-YP801619FGB
CSB-EP801619FGB
CSB-BP801619FGB
CSB-MP801619FGB
CSB-EP801619FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP801620FGB
CSB-EP801620FGB
CSB-BP801620FGB
CSB-MP801620FGB
CSB-EP801620FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ornithine carbamoyltransferase, catabolic (arcB) CSB-YP801621FGB
CSB-EP801621FGB
CSB-BP801621FGB
CSB-MP801621FGB
CSB-EP801621FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP801622FGB
CSB-EP801622FGB
CSB-BP801622FGB
CSB-MP801622FGB
CSB-EP801622FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP801623FGB
CSB-EP801623FGB
CSB-BP801623FGB
CSB-MP801623FGB
CSB-EP801623FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida PKHD-type hydroxylase PP_0862 (PP_0862) CSB-YP801624FGB
CSB-EP801624FGB
CSB-BP801624FGB
CSB-MP801624FGB
CSB-EP801624FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Cysteine desulfurase (iscS) CSB-YP801625FGB
CSB-EP801625FGB
CSB-BP801625FGB
CSB-MP801625FGB
CSB-EP801625FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Flavohemoprotein (hmp) CSB-YP801626FGB
CSB-EP801626FGB
CSB-BP801626FGB
CSB-MP801626FGB
CSB-EP801626FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ferrochelatase (hemH) CSB-YP801627FGB
CSB-EP801627FGB
CSB-BP801627FGB
CSB-MP801627FGB
CSB-EP801627FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida GTPase obg (obg) CSB-YP801628FGB
CSB-EP801628FGB
CSB-BP801628FGB
CSB-MP801628FGB
CSB-EP801628FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA gyrase inhibitor YacG (yacG) CSB-YP801629FGB
CSB-EP801629FGB
CSB-BP801629FGB
CSB-MP801629FGB
CSB-EP801629FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Biosynthetic arginine decarboxylase (speA), partial CSB-YP801630FGB
CSB-EP801630FGB
CSB-BP801630FGB
CSB-MP801630FGB
CSB-EP801630FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP801631FGB
CSB-EP801631FGB
CSB-BP801631FGB
CSB-MP801631FGB
CSB-EP801631FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S13 (rpsM) CSB-YP801632FGB
CSB-EP801632FGB
CSB-BP801632FGB
CSB-MP801632FGB
CSB-EP801632FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S8 (rpsH) CSB-YP801633FGB
CSB-EP801633FGB
CSB-BP801633FGB
CSB-MP801633FGB
CSB-EP801633FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S17 (rpsQ) CSB-YP801634FGB
CSB-EP801634FGB
CSB-BP801634FGB
CSB-MP801634FGB
CSB-EP801634FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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