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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Tropheryma whipplei Non-canonical purine NTP pyrophosphatase (TW639) CSB-YP802774TIV
CSB-EP802774TIV
CSB-BP802774TIV
CSB-MP802774TIV
CSB-EP802774TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP802775TIV
CSB-EP802775TIV
CSB-BP802775TIV
CSB-MP802775TIV
CSB-EP802775TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Glutamate--tRNA ligase (gltX) CSB-YP802776TIV
CSB-EP802776TIV
CSB-BP802776TIV
CSB-MP802776TIV
CSB-EP802776TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Threonine--tRNA ligase (thrS), partial CSB-YP802777TIV
CSB-EP802777TIV
CSB-BP802777TIV
CSB-MP802777TIV
CSB-EP802777TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Probable rRNA maturation factor (TW489) CSB-YP802778TIV
CSB-EP802778TIV
CSB-BP802778TIV
CSB-MP802778TIV
CSB-EP802778TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L31 type B (rpmE2) CSB-YP802779TIV
CSB-EP802779TIV
CSB-BP802779TIV
CSB-MP802779TIV
CSB-EP802779TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 3-dehydroquinate synthase (aroB) CSB-YP802780TIV
CSB-EP802780TIV
CSB-BP802780TIV
CSB-MP802780TIV
CSB-EP802780TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP802781TIV
CSB-EP802781TIV
CSB-BP802781TIV
CSB-MP802781TIV
CSB-EP802781TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP802782TIV
CSB-EP802782TIV
CSB-BP802782TIV
CSB-MP802782TIV
CSB-EP802782TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei ATP synthase subunit b (atpF), partial CSB-YP802783TIV1
CSB-EP802783TIV1
CSB-BP802783TIV1
CSB-MP802783TIV1
CSB-EP802783TIV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP802784TIV
CSB-EP802784TIV
CSB-BP802784TIV
CSB-MP802784TIV
CSB-EP802784TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP802785TIV
CSB-EP802785TIV
CSB-BP802785TIV
CSB-MP802785TIV
CSB-EP802785TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L36 (rpmJ) CSB-YP802786TIV
CSB-EP802786TIV
CSB-BP802786TIV
CSB-MP802786TIV
CSB-EP802786TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L18 (rplR) CSB-YP802787TIV
CSB-EP802787TIV
CSB-BP802787TIV
CSB-MP802787TIV
CSB-EP802787TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 30S ribosomal protein S3 (rpsC) CSB-YP802788TIV
CSB-EP802788TIV
CSB-BP802788TIV
CSB-MP802788TIV
CSB-EP802788TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Phosphopantetheine adenylyltransferase (coaD) CSB-YP802789TIV
CSB-EP802789TIV
CSB-BP802789TIV
CSB-MP802789TIV
CSB-EP802789TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP802790TIV
CSB-EP802790TIV
CSB-BP802790TIV
CSB-MP802790TIV
CSB-EP802790TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP802791TIV
CSB-EP802791TIV
CSB-BP802791TIV
CSB-MP802791TIV
CSB-EP802791TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglycerol transferase I (mdoB), partial CSB-YP802792SZB1
CSB-EP802792SZB1
CSB-BP802792SZB1
CSB-MP802792SZB1
CSB-EP802792SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltoporin (lamB) CSB-YP802793SZB
CSB-EP802793SZB
CSB-BP802793SZB
CSB-MP802793SZB
CSB-EP802793SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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