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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Oxoglutarate dehydrogenase (sucA), partial CSB-YP804620BMZ
CSB-EP804620BMZ
CSB-BP804620BMZ
CSB-MP804620BMZ
CSB-EP804620BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Inositol-1-monophosphatase (suhB) CSB-YP804621BMZ
CSB-EP804621BMZ
CSB-BP804621BMZ
CSB-MP804621BMZ
CSB-EP804621BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP804622BMZ
CSB-EP804622BMZ
CSB-BP804622BMZ
CSB-MP804622BMZ
CSB-EP804622BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae SsrA-binding protein (smpB) CSB-YP804623BMZ
CSB-EP804623BMZ
CSB-BP804623BMZ
CSB-MP804623BMZ
CSB-EP804623BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Proline--tRNA ligase (proS), partial CSB-YP804624BMZ
CSB-EP804624BMZ
CSB-BP804624BMZ
CSB-MP804624BMZ
CSB-EP804624BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Acetolactate synthase small subunit (ilvH) CSB-YP804625BMZ
CSB-EP804625BMZ
CSB-BP804625BMZ
CSB-MP804625BMZ
CSB-EP804625BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP804626BMZ
CSB-EP804626BMZ
CSB-BP804626BMZ
CSB-MP804626BMZ
CSB-EP804626BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Bifunctional aspartokinase/homoserine dehydrogenase (thrA), partial CSB-YP804627BMZ
CSB-EP804627BMZ
CSB-BP804627BMZ
CSB-MP804627BMZ
CSB-EP804627BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Glutaredoxin-4 (grxD) CSB-YP804628BMZ
CSB-EP804628BMZ
CSB-BP804628BMZ
CSB-MP804628BMZ
CSB-EP804628BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized ferredoxin-like protein bbp_166 (bbp_166) CSB-YP804629BMZ
CSB-EP804629BMZ
CSB-BP804629BMZ
CSB-MP804629BMZ
CSB-EP804629BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized membrane protein bbp_156 (bbp_156), partial CSB-YP804630BMZ
CSB-EP804630BMZ
CSB-BP804630BMZ
CSB-MP804630BMZ
CSB-EP804630BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP804631BMZ
CSB-EP804631BMZ
CSB-BP804631BMZ
CSB-MP804631BMZ
CSB-EP804631BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Chaperone protein DnaJ (dnaJ) CSB-YP804632BMZ
CSB-EP804632BMZ
CSB-BP804632BMZ
CSB-MP804632BMZ
CSB-EP804632BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Electron transport complex protein RnfE (rnfE), partial CSB-YP804633BMZ1
CSB-EP804633BMZ1
CSB-BP804633BMZ1
CSB-MP804633BMZ1
CSB-EP804633BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae tRNA (Ile)-lysidine synthase (tilS) CSB-YP804634BMZ
CSB-EP804634BMZ
CSB-BP804634BMZ
CSB-MP804634BMZ
CSB-EP804634BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Chorismate synthase (aroC) CSB-YP804635BMZ
CSB-EP804635BMZ
CSB-BP804635BMZ
CSB-MP804635BMZ
CSB-EP804635BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Protein pmbA homolog (pmbA) CSB-YP804636BMZ
CSB-EP804636BMZ
CSB-BP804636BMZ
CSB-MP804636BMZ
CSB-EP804636BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar motor switch protein FliM (fliM) CSB-YP804637BMZ
CSB-EP804637BMZ
CSB-BP804637BMZ
CSB-MP804637BMZ
CSB-EP804637BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar hook-basal body complex protein FliE (fliE) CSB-YP804638BMZ
CSB-EP804638BMZ
CSB-BP804638BMZ
CSB-MP804638BMZ
CSB-EP804638BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae DNA primase (dnaG), partial CSB-YP804639BMZ
CSB-EP804639BMZ
CSB-BP804639BMZ
CSB-MP804639BMZ
CSB-EP804639BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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