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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP802714DXP
CSB-EP802714DXP
CSB-BP802714DXP
CSB-MP802714DXP
CSB-EP802714DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Transcription elongation protein nusA (nusA), partial CSB-YP802715DXP
CSB-EP802715DXP
CSB-BP802715DXP
CSB-MP802715DXP
CSB-EP802715DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Uncharacterized HTH-type transcriptional regulator CBU_1416 (CBU_1416) CSB-YP802716DXP
CSB-EP802716DXP
CSB-BP802716DXP
CSB-MP802716DXP
CSB-EP802716DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP802717DXP
CSB-EP802717DXP
CSB-BP802717DXP
CSB-MP802717DXP
CSB-EP802717DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Integration host factor subunit alpha (ihfA) CSB-YP802718DXP
CSB-EP802718DXP
CSB-BP802718DXP
CSB-MP802718DXP
CSB-EP802718DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Nucleoside diphosphate kinase (ndk) CSB-YP802719DXP
CSB-EP802719DXP
CSB-BP802719DXP
CSB-MP802719DXP
CSB-EP802719DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Oligoribonuclease (orn) CSB-YP802720DXP
CSB-EP802720DXP
CSB-BP802720DXP
CSB-MP802720DXP
CSB-EP802720DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Tryptophan synthase alpha chain (trpA) CSB-YP802721DXP
CSB-EP802721DXP
CSB-BP802721DXP
CSB-MP802721DXP
CSB-EP802721DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA mismatch repair protein mutL (mutL), partial CSB-YP802722DXP
CSB-EP802722DXP
CSB-BP802722DXP
CSB-MP802722DXP
CSB-EP802722DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Carbon storage regulator homolog 2 (csrA2) CSB-YP802723DXP
CSB-EP802723DXP
CSB-BP802723DXP
CSB-MP802723DXP
CSB-EP802723DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii UPF0422 protein CBU_0937 CSB-YP802724DXP
CSB-EP802724DXP
CSB-BP802724DXP
CSB-MP802724DXP
CSB-EP802724DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP802725DXP
CSB-EP802725DXP
CSB-BP802725DXP
CSB-MP802725DXP
CSB-EP802725DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 30S ribosomal protein S18 (rpsR) CSB-YP802726DXP
CSB-EP802726DXP
CSB-BP802726DXP
CSB-MP802726DXP
CSB-EP802726DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glucose-6-phosphate isomerase (pgi), partial CSB-YP802727DXP
CSB-EP802727DXP
CSB-BP802727DXP
CSB-MP802727DXP
CSB-EP802727DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP802728DXP
CSB-EP802728DXP
CSB-BP802728DXP
CSB-MP802728DXP
CSB-EP802728DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Recombination protein RecR (recR) CSB-YP802729DXP
CSB-EP802729DXP
CSB-BP802729DXP
CSB-MP802729DXP
CSB-EP802729DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Thiosulfate sulfurtransferase glpE (glpE) CSB-YP802730DXP
CSB-EP802730DXP
CSB-BP802730DXP
CSB-MP802730DXP
CSB-EP802730DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA ligase (ligA), partial CSB-YP802731DXP
CSB-EP802731DXP
CSB-BP802731DXP
CSB-MP802731DXP
CSB-EP802731DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphate acyltransferase (plsX) CSB-YP802732DXP
CSB-EP802732DXP
CSB-BP802732DXP
CSB-MP802732DXP
CSB-EP802732DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosome maturation factor RimM (rimM) CSB-YP802733DXP
CSB-EP802733DXP
CSB-BP802733DXP
CSB-MP802733DXP
CSB-EP802733DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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