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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas syringae pv. tomato DNA mismatch repair protein MutS (mutS), partial CSB-YP803375FGP
CSB-EP803375FGP
CSB-BP803375FGP
CSB-MP803375FGP
CSB-EP803375FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato DNA polymerase IV (dinB) CSB-YP803376FGP
CSB-EP803376FGP
CSB-BP803376FGP
CSB-MP803376FGP
CSB-EP803376FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 7-cyano-7-deazaguanine synthase (queC) CSB-YP803377FGP
CSB-EP803377FGP
CSB-BP803377FGP
CSB-MP803377FGP
CSB-EP803377FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable M18 family aminopeptidase 2 (apeB) CSB-YP803378FGP
CSB-EP803378FGP
CSB-BP803378FGP
CSB-MP803378FGP
CSB-EP803378FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Maf-like protein PSPTO_3837 (maf-1) CSB-YP803379FGP
CSB-EP803379FGP
CSB-BP803379FGP
CSB-MP803379FGP
CSB-EP803379FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0059 membrane protein PSPTO_3755 (PSPTO_3755), partial CSB-YP803380FGP1
CSB-EP803380FGP1
CSB-BP803380FGP1
CSB-MP803380FGP1
CSB-EP803380FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP803381FGP
CSB-EP803381FGP
CSB-BP803381FGP
CSB-MP803381FGP
CSB-EP803381FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Cell division protein ZipA homolog (zipA), partial CSB-YP803382FGP
CSB-EP803382FGP
CSB-BP803382FGP
CSB-MP803382FGP
CSB-EP803382FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP803383FGP
CSB-EP803383FGP
CSB-BP803383FGP
CSB-MP803383FGP
CSB-EP803383FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Alkanesulfonate monooxygenase (ssuD) CSB-YP803384FGP
CSB-EP803384FGP
CSB-BP803384FGP
CSB-MP803384FGP
CSB-EP803384FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato NADH-quinone oxidoreductase subunit G (nuoG), partial CSB-YP803385FGP
CSB-EP803385FGP
CSB-BP803385FGP
CSB-MP803385FGP
CSB-EP803385FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP803386FGP
CSB-EP803386FGP
CSB-BP803386FGP
CSB-MP803386FGP
CSB-EP803386FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Outer-membrane lipoprotein carrier protein (lolA) CSB-YP803387FGP
CSB-EP803387FGP
CSB-BP803387FGP
CSB-MP803387FGP
CSB-EP803387FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato FMN-dependent NADH-azoreductase 1 (azoR1) CSB-YP803388FGP
CSB-EP803388FGP
CSB-BP803388FGP
CSB-MP803388FGP
CSB-EP803388FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spodoptera littoralis nuclear polyhedrosis virus Ecdysteroid UDP-glucosyltransferase (EGT) CSB-YP803389FKR
CSB-EP803389FKR
CSB-BP803389FKR
CSB-MP803389FKR
CSB-EP803389FKR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Error-prone DNA polymerase (dnaE2), partial CSB-YP803390FGP
CSB-EP803390FGP
CSB-BP803390FGP
CSB-MP803390FGP
CSB-EP803390FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato NADH pyrophosphatase (nudC) CSB-YP803391FGP
CSB-EP803391FGP
CSB-BP803391FGP
CSB-MP803391FGP
CSB-EP803391FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Urease accessory protein UreE 1 (ureE1) CSB-YP803392FGP
CSB-EP803392FGP
CSB-BP803392FGP
CSB-MP803392FGP
CSB-EP803392FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Integration host factor subunit alpha (ihfA) CSB-YP803393FGP
CSB-EP803393FGP
CSB-BP803393FGP
CSB-MP803393FGP
CSB-EP803393FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Molybdenum cofactor guanylyltransferase (mobA) CSB-YP803394FGP
CSB-EP803394FGP
CSB-BP803394FGP
CSB-MP803394FGP
CSB-EP803394FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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