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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L5 (rplE) CSB-YP803610CMN
CSB-EP803610CMN
CSB-BP803610CMN
CSB-MP803610CMN
CSB-EP803610CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S11 (rpsK) CSB-YP803611CMN
CSB-EP803611CMN
CSB-BP803611CMN
CSB-MP803611CMN
CSB-EP803611CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Butyrate kinase (buk) CSB-YP803612CMN
CSB-EP803612CMN
CSB-BP803612CMN
CSB-MP803612CMN
CSB-EP803612CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein C (uvrC), partial CSB-YP803613CMN
CSB-EP803613CMN
CSB-BP803613CMN
CSB-MP803613CMN
CSB-EP803613CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A51 (MVA164R, ACAM3000_MVA_164) CSB-YP803614VEE
CSB-EP803614VEE
CSB-BP803614VEE
CSB-MP803614VEE
CSB-EP803614VEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan--tRNA ligase (trpS) CSB-YP803615CMN
CSB-EP803615CMN
CSB-BP803615CMN
CSB-MP803615CMN
CSB-EP803615CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0178 protein CTC_02403 (CTC_02403) CSB-YP803616CMN
CSB-EP803616CMN
CSB-BP803616CMN
CSB-MP803616CMN
CSB-EP803616CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orotate phosphoribosyltransferase (pyrE) CSB-YP803617CMN
CSB-EP803617CMN
CSB-BP803617CMN
CSB-MP803617CMN
CSB-EP803617CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant V-type ATP synthase subunit E 2 (atpE2) CSB-YP803618CMN
CSB-EP803618CMN
CSB-BP803618CMN
CSB-MP803618CMN
CSB-EP803618CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP803619CMN
CSB-EP803619CMN
CSB-BP803619CMN
CSB-MP803619CMN
CSB-EP803619CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant MutS2 protein (mutS2), partial CSB-YP803620CMN
CSB-EP803620CMN
CSB-BP803620CMN
CSB-MP803620CMN
CSB-EP803620CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP803621CMN
CSB-EP803621CMN
CSB-BP803621CMN
CSB-MP803621CMN
CSB-EP803621CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP803622CMN
CSB-EP803622CMN
CSB-BP803622CMN
CSB-MP803622CMN
CSB-EP803622CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable septum site-determining protein MinC (minC) CSB-YP803623CMN
CSB-EP803623CMN
CSB-BP803623CMN
CSB-MP803623CMN
CSB-EP803623CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP803624CMN
CSB-EP803624CMN
CSB-BP803624CMN
CSB-MP803624CMN
CSB-EP803624CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zucchini yellow mosaic virus Genome polyprotein, partial CSB-YP803625ZAP
CSB-EP803625ZAP
CSB-BP803625ZAP
CSB-MP803625ZAP
CSB-EP803625ZAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiazole synthase (thiG) CSB-YP803626CMN
CSB-EP803626CMN
CSB-BP803626CMN
CSB-MP803626CMN
CSB-EP803626CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Virion infectivity factor (vif) CSB-YP803627HDAK
CSB-EP803627HDAK
CSB-BP803627HDAK
CSB-MP803627HDAK
CSB-EP803627HDAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein MraZ (mraZ) CSB-YP803628CMN
CSB-EP803628CMN
CSB-BP803628CMN
CSB-MP803628CMN
CSB-EP803628CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate synthase (aroB) CSB-YP803629CMN
CSB-EP803629CMN
CSB-BP803629CMN
CSB-MP803629CMN
CSB-EP803629CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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