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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L10 (rplJ) CSB-YP804640BMZ
CSB-EP804640BMZ
CSB-BP804640BMZ
CSB-MP804640BMZ
CSB-EP804640BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP804641BMZ
CSB-EP804641BMZ
CSB-BP804641BMZ
CSB-MP804641BMZ
CSB-EP804641BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae DNA polymerase III subunit beta (dnaN) CSB-YP804642BMZ
CSB-EP804642BMZ
CSB-BP804642BMZ
CSB-MP804642BMZ
CSB-EP804642BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae ATP synthase subunit b (atpF), partial CSB-YP804643BMZ1
CSB-EP804643BMZ1
CSB-BP804643BMZ1
CSB-MP804643BMZ1
CSB-EP804643BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Probable replication-associated protein repA1 (repA1) CSB-YP804644BMZ
CSB-EP804644BMZ
CSB-BP804644BMZ
CSB-MP804644BMZ
CSB-EP804644BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Peptide deformylase (def) CSB-YP804645BVW
CSB-EP804645BVW
CSB-BP804645BVW
CSB-MP804645BVW
CSB-EP804645BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glucans biosynthesis glucosyltransferase H (opgH), partial CSB-YP804646BVW1
CSB-EP804646BVW1
CSB-BP804646BVW1
CSB-MP804646BVW1
CSB-EP804646BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Flavoprotein WrbA (wrbA) CSB-YP804647BVW
CSB-EP804647BVW
CSB-BP804647BVW
CSB-MP804647BVW
CSB-EP804647BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 6,7-dimethyl-8-ribityllumazine synthase 2 (ribH2) CSB-YP804648BVW
CSB-EP804648BVW
CSB-BP804648BVW
CSB-MP804648BVW
CSB-EP804648BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Carbamoyl-phosphate synthase small chain (carA) CSB-YP804649BVW
CSB-EP804649BVW
CSB-BP804649BVW
CSB-MP804649BVW
CSB-EP804649BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum C4-dicarboxylate transport protein 4 (dctA4), partial CSB-YP804650BVW1
CSB-EP804650BVW1
CSB-BP804650BVW1
CSB-MP804650BVW1
CSB-EP804650BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Alkanesulfonate monooxygenase (ssuD) CSB-YP804651BVW
CSB-EP804651BVW
CSB-BP804651BVW
CSB-MP804651BVW
CSB-EP804651BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Flagellar P-ring protein 2 (flgI2) CSB-YP804652BVW
CSB-EP804652BVW
CSB-BP804652BVW
CSB-MP804652BVW
CSB-EP804652BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Pyrroloquinoline-quinone synthase (pqqC) CSB-YP804653BVW
CSB-EP804653BVW
CSB-BP804653BVW
CSB-MP804653BVW
CSB-EP804653BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP804654BVW
CSB-EP804654BVW
CSB-BP804654BVW
CSB-MP804654BVW
CSB-EP804654BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ribosomal protein L11 methyltransferase (prmA) CSB-YP804655BVW
CSB-EP804655BVW
CSB-BP804655BVW
CSB-MP804655BVW
CSB-EP804655BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Amino acid--[acyl-carrier-protein] ligase 2 (bll6282) CSB-YP804656BVW
CSB-EP804656BVW
CSB-BP804656BVW
CSB-MP804656BVW
CSB-EP804656BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Dihydroxy-acid dehydratase 3 (ilvD3), partial CSB-YP804657BVW
CSB-EP804657BVW
CSB-BP804657BVW
CSB-MP804657BVW
CSB-EP804657BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable flagellum biosynthesis repressor protein flbT 1 (flbT1) CSB-YP804658BVW
CSB-EP804658BVW
CSB-BP804658BVW
CSB-MP804658BVW
CSB-EP804658BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP804659BVW
CSB-EP804659BVW
CSB-BP804659BVW
CSB-MP804659BVW
CSB-EP804659BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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