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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Chaperone protein htpG (htpG), partial CSB-YP803670BMZ
CSB-EP803670BMZ
CSB-BP803670BMZ
CSB-MP803670BMZ
CSB-EP803670BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP803671BMZ
CSB-EP803671BMZ
CSB-BP803671BMZ
CSB-MP803671BMZ
CSB-EP803671BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Sulfurtransferase TusE (tusE) CSB-YP803672BMZ
CSB-EP803672BMZ
CSB-BP803672BMZ
CSB-MP803672BMZ
CSB-EP803672BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae UPF0056 membrane protein bbp_399 (bbp_399), partial CSB-YP803673BMZ1
CSB-EP803673BMZ1
CSB-BP803673BMZ1
CSB-MP803673BMZ1
CSB-EP803673BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Peptide chain release factor 2 (prfB) CSB-YP803674BMZ
CSB-EP803674BMZ
CSB-BP803674BMZ
CSB-MP803674BMZ
CSB-EP803674BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Glutamine--tRNA ligase (glnS), partial CSB-YP803675BMZ
CSB-EP803675BMZ
CSB-BP803675BMZ
CSB-MP803675BMZ
CSB-EP803675BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae P-protein (pheA) CSB-YP803676BMZ
CSB-EP803676BMZ
CSB-BP803676BMZ
CSB-MP803676BMZ
CSB-EP803676BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP803677BMZ
CSB-EP803677BMZ
CSB-BP803677BMZ
CSB-MP803677BMZ
CSB-EP803677BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Cold-shock DEAD box protein A homolog (deaD), partial CSB-YP803678BMZ
CSB-EP803678BMZ
CSB-BP803678BMZ
CSB-MP803678BMZ
CSB-EP803678BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae DNA polymerase III subunit delta' (holB) CSB-YP803679BMZ
CSB-EP803679BMZ
CSB-BP803679BMZ
CSB-MP803679BMZ
CSB-EP803679BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Putative truncated ribonuclease E (rne) CSB-YP803680BMZ
CSB-EP803680BMZ
CSB-BP803680BMZ
CSB-MP803680BMZ
CSB-EP803680BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Cell division topological specificity factor (minE) CSB-YP803681BMZ
CSB-EP803681BMZ
CSB-BP803681BMZ
CSB-MP803681BMZ
CSB-EP803681BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 30S ribosomal protein S1 (rpsA), partial CSB-YP803682BMZ
CSB-EP803682BMZ
CSB-BP803682BMZ
CSB-MP803682BMZ
CSB-EP803682BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Deoxyribodipyrimidine photo-lyase (phrB) CSB-YP803683BMZ
CSB-EP803683BMZ
CSB-BP803683BMZ
CSB-MP803683BMZ
CSB-EP803683BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 8-amino-7-oxononanoate synthase (bioF) CSB-YP803684BMZ
CSB-EP803684BMZ
CSB-BP803684BMZ
CSB-MP803684BMZ
CSB-EP803684BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Lipoyl synthase (lipA) CSB-YP803685BMZ
CSB-EP803685BMZ
CSB-BP803685BMZ
CSB-MP803685BMZ
CSB-EP803685BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Elongation factor 4 (lepA), partial CSB-YP803686BMZ
CSB-EP803686BMZ
CSB-BP803686BMZ
CSB-MP803686BMZ
CSB-EP803686BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae DNA polymerase III subunit epsilon (dnaQ) CSB-YP803687BMZ
CSB-EP803687BMZ
CSB-BP803687BMZ
CSB-MP803687BMZ
CSB-EP803687BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Undecaprenyl pyrophosphate synthase (uppS) CSB-YP803688BMZ
CSB-EP803688BMZ
CSB-BP803688BMZ
CSB-MP803688BMZ
CSB-EP803688BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Probable periplasmic serine endoprotease DegP-like (htrA) CSB-YP803689BMZ
CSB-EP803689BMZ
CSB-BP803689BMZ
CSB-MP803689BMZ
CSB-EP803689BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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