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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida DNA replication and repair protein recF (recF) CSB-YP803535FGB
CSB-EP803535FGB
CSB-BP803535FGB
CSB-MP803535FGB
CSB-EP803535FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum D-ribose pyranase (rbsD) CSB-YP803536LMS
CSB-EP803536LMS
CSB-BP803536LMS
CSB-MP803536LMS
CSB-EP803536LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum L-rhamnose isomerase (rhaA) CSB-YP803537LMS
CSB-EP803537LMS
CSB-BP803537LMS
CSB-MP803537LMS
CSB-EP803537LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum N-acetylmuramic acid 6-phosphate etherase 2 (murQ2) CSB-YP803538LMS
CSB-EP803538LMS
CSB-BP803538LMS
CSB-MP803538LMS
CSB-EP803538LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Macro domain-containing protein lp_3408 (lp_3408) CSB-YP803539LMS
CSB-EP803539LMS
CSB-BP803539LMS
CSB-MP803539LMS
CSB-EP803539LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Adenylosuccinate synthetase (purA) CSB-YP803540LMS
CSB-EP803540LMS
CSB-BP803540LMS
CSB-MP803540LMS
CSB-EP803540LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum UPF0324 membrane protein lp_2841 (lp_2841), partial CSB-YP803541LMS
CSB-EP803541LMS
CSB-BP803541LMS
CSB-MP803541LMS
CSB-EP803541LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP803542LMS
CSB-EP803542LMS
CSB-BP803542LMS
CSB-MP803542LMS
CSB-EP803542LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP803543LMS
CSB-EP803543LMS
CSB-BP803543LMS
CSB-MP803543LMS
CSB-EP803543LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP803544LMS
CSB-EP803544LMS
CSB-BP803544LMS
CSB-MP803544LMS
CSB-EP803544LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum ATP synthase subunit delta (atpH) CSB-YP803545LMS
CSB-EP803545LMS
CSB-BP803545LMS
CSB-MP803545LMS
CSB-EP803545LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum UPF0637 protein lp_2332 (lp_2332) CSB-YP803546LMS
CSB-EP803546LMS
CSB-BP803546LMS
CSB-MP803546LMS
CSB-EP803546LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Protein RecA (recA) CSB-YP803547LMS
CSB-EP803547LMS
CSB-BP803547LMS
CSB-MP803547LMS
CSB-EP803547LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum UPF0297 protein lp_2275 (lp_2275) CSB-YP803548LMS
CSB-EP803548LMS
CSB-BP803548LMS
CSB-MP803548LMS
CSB-EP803548LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum MutS2 protein (mutS2), partial CSB-YP803549LMS
CSB-EP803549LMS
CSB-BP803549LMS
CSB-MP803549LMS
CSB-EP803549LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP803550LMS
CSB-EP803550LMS
CSB-BP803550LMS
CSB-MP803550LMS
CSB-EP803550LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP803551LMS
CSB-EP803551LMS
CSB-BP803551LMS
CSB-MP803551LMS
CSB-EP803551LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Ribose-phosphate pyrophosphokinase 2 (prs2) CSB-YP803552LMS
CSB-EP803552LMS
CSB-BP803552LMS
CSB-MP803552LMS
CSB-EP803552LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum 30S ribosomal protein S15 (rpsO) CSB-YP803553LMS
CSB-EP803553LMS
CSB-BP803553LMS
CSB-MP803553LMS
CSB-EP803553LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Probable GTP-binding protein EngB (engB) CSB-YP803554LMS
CSB-EP803554LMS
CSB-BP803554LMS
CSB-MP803554LMS
CSB-EP803554LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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