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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium japonicum Flagellar hook-basal body complex protein FliE 2 (fliE2) CSB-YP804184BVW
CSB-EP804184BVW
CSB-BP804184BVW
CSB-MP804184BVW
CSB-EP804184BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Coenzyme PQQ synthesis protein B (pqqB) CSB-YP804185BVW
CSB-EP804185BVW
CSB-BP804185BVW
CSB-MP804185BVW
CSB-EP804185BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP804186BVW
CSB-EP804186BVW
CSB-BP804186BVW
CSB-MP804186BVW
CSB-EP804186BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0391 membrane protein bsl6560 (bsl6560), partial CSB-YP804187BVW
CSB-EP804187BVW
CSB-BP804187BVW
CSB-MP804187BVW
CSB-EP804187BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP804188BVW
CSB-EP804188BVW
CSB-BP804188BVW
CSB-MP804188BVW
CSB-EP804188BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Imidazolonepropionase (hutI) CSB-YP804189BVW
CSB-EP804189BVW
CSB-BP804189BVW
CSB-MP804189BVW
CSB-EP804189BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Bifunctional protein HldE (hldE) CSB-YP804190BVW
CSB-EP804190BVW
CSB-BP804190BVW
CSB-MP804190BVW
CSB-EP804190BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP804191BVW
CSB-EP804191BVW
CSB-BP804191BVW
CSB-MP804191BVW
CSB-EP804191BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP804192BVW
CSB-EP804192BVW
CSB-BP804192BVW
CSB-MP804192BVW
CSB-EP804192BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L4 (rplD) CSB-YP804193BVW
CSB-EP804193BVW
CSB-BP804193BVW
CSB-MP804193BVW
CSB-EP804193BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S3 (rpsC) CSB-YP804194BVW
CSB-EP804194BVW
CSB-BP804194BVW
CSB-MP804194BVW
CSB-EP804194BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L6 (rplF) CSB-YP804195BVW
CSB-EP804195BVW
CSB-BP804195BVW
CSB-MP804195BVW
CSB-EP804195BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L17 (rplQ) CSB-YP804196BVW
CSB-EP804196BVW
CSB-BP804196BVW
CSB-MP804196BVW
CSB-EP804196BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Aspartate carbamoyltransferase (pyrB) CSB-YP804197BVW
CSB-EP804197BVW
CSB-BP804197BVW
CSB-MP804197BVW
CSB-EP804197BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum K (+)-insensitive pyrophosphate-energized proton pump (hppA), partial CSB-YP804198BVW
CSB-EP804198BVW
CSB-BP804198BVW
CSB-MP804198BVW
CSB-EP804198BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP804199BVW
CSB-EP804199BVW
CSB-BP804199BVW
CSB-MP804199BVW
CSB-EP804199BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S2 (rpsB) CSB-YP804200BVW
CSB-EP804200BVW
CSB-BP804200BVW
CSB-MP804200BVW
CSB-EP804200BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glutamine--tRNA ligase (glnS), partial CSB-YP804201BVW
CSB-EP804201BVW
CSB-BP804201BVW
CSB-MP804201BVW
CSB-EP804201BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Sec-independent protein translocase protein TatB (tatB), partial CSB-YP804202BVW
CSB-EP804202BVW
CSB-BP804202BVW
CSB-MP804202BVW
CSB-EP804202BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 60 kDa chaperonin 5 (groL5), partial CSB-YP804203BVW
CSB-EP804203BVW
CSB-BP804203BVW
CSB-MP804203BVW
CSB-EP804203BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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