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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus plantarum Phosphonoacetaldehyde hydrolase (phnX) CSB-YP804064LMS
CSB-EP804064LMS
CSB-BP804064LMS
CSB-MP804064LMS
CSB-EP804064LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum UPF0133 protein lp_0699 (lp_0699) CSB-YP804065LMS
CSB-EP804065LMS
CSB-BP804065LMS
CSB-MP804065LMS
CSB-EP804065LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Glutamate--tRNA ligase (gltX) CSB-YP804066LMS
CSB-EP804066LMS
CSB-BP804066LMS
CSB-MP804066LMS
CSB-EP804066LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP804067LMS
CSB-EP804067LMS
CSB-BP804067LMS
CSB-MP804067LMS
CSB-EP804067LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Protease HtpX homolog (htpX), partial CSB-YP804068LMS1
CSB-EP804068LMS1
CSB-BP804068LMS1
CSB-MP804068LMS1
CSB-EP804068LMS1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Bifunctional protein GlmU (glmU) CSB-YP804069LMS
CSB-EP804069LMS
CSB-BP804069LMS
CSB-MP804069LMS
CSB-EP804069LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Teichoic acids export ATP-binding protein TagH (tagH) CSB-YP804070LMS
CSB-EP804070LMS
CSB-BP804070LMS
CSB-MP804070LMS
CSB-EP804070LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Thiamine-phosphate synthase (thiE) CSB-YP804071LMS
CSB-EP804071LMS
CSB-BP804071LMS
CSB-MP804071LMS
CSB-EP804071LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP804072LMS
CSB-EP804072LMS
CSB-BP804072LMS
CSB-MP804072LMS
CSB-EP804072LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum 30S ribosomal protein S6 (rpsF) CSB-YP804073LMS
CSB-EP804073LMS
CSB-BP804073LMS
CSB-MP804073LMS
CSB-EP804073LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP804074CMN
CSB-EP804074CMN
CSB-BP804074CMN
CSB-MP804074CMN
CSB-EP804074CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP804075CMN
CSB-EP804075CMN
CSB-BP804075CMN
CSB-MP804075CMN
CSB-EP804075CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L16 (rplP) CSB-YP804076CMN
CSB-EP804076CMN
CSB-BP804076CMN
CSB-MP804076CMN
CSB-EP804076CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP804077CMN
CSB-EP804077CMN
CSB-BP804077CMN
CSB-MP804077CMN
CSB-EP804077CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S9 (rpsI) CSB-YP804078CMN
CSB-EP804078CMN
CSB-BP804078CMN
CSB-MP804078CMN
CSB-EP804078CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein A (uvrA), partial CSB-YP804079CMN
CSB-EP804079CMN
CSB-BP804079CMN
CSB-MP804079CMN
CSB-EP804079CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus Protein A40 homolog (A45R), partial CSB-YP804080VAR
CSB-EP804080VAR
CSB-BP804080VAR
CSB-MP804080VAR
CSB-EP804080VAR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP804081CMN
CSB-EP804081CMN
CSB-BP804081CMN
CSB-MP804081CMN
CSB-EP804081CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthine phosphoribosyltransferase (xpt) CSB-YP804082CMN
CSB-EP804082CMN
CSB-BP804082CMN
CSB-MP804082CMN
CSB-EP804082CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP804083CMN
CSB-EP804083CMN
CSB-BP804083CMN
CSB-MP804083CMN
CSB-EP804083CMN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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