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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus plantarum Proline iminopeptidase (pip) CSB-YP804540LMS
CSB-EP804540LMS
CSB-BP804540LMS
CSB-MP804540LMS
CSB-EP804540LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Glutamate 5-kinase (proB) CSB-YP804541LMS
CSB-EP804541LMS
CSB-BP804541LMS
CSB-MP804541LMS
CSB-EP804541LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ATP:guanido phosphotransferase CTC_02634 (CTC_02634) CSB-YP804542CMN
CSB-EP804542CMN
CSB-BP804542CMN
CSB-MP804542CMN
CSB-EP804542CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate synthase thyX (thyX) CSB-YP804543CMN
CSB-EP804543CMN
CSB-BP804543CMN
CSB-MP804543CMN
CSB-EP804543CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S7 (rpsG) CSB-YP804544CMN
CSB-EP804544CMN
CSB-BP804544CMN
CSB-MP804544CMN
CSB-EP804544CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP804545CMN
CSB-EP804545CMN
CSB-BP804545CMN
CSB-MP804545CMN
CSB-EP804545CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L15 (rplO) CSB-YP804546CMN
CSB-EP804546CMN
CSB-BP804546CMN
CSB-MP804546CMN
CSB-EP804546CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP804547CMN
CSB-EP804547CMN
CSB-BP804547CMN
CSB-MP804547CMN
CSB-EP804547CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein B (uvrB), partial CSB-YP804548CMN
CSB-EP804548CMN
CSB-BP804548CMN
CSB-MP804548CMN
CSB-EP804548CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6-phosphofructokinase (pfkA) CSB-YP804549CMN
CSB-EP804549CMN
CSB-BP804549CMN
CSB-MP804549CMN
CSB-EP804549CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Redox-sensing transcriptional repressor rex (rex) CSB-YP804550CMN
CSB-EP804550CMN
CSB-BP804550CMN
CSB-MP804550CMN
CSB-EP804550CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP804551CMN
CSB-EP804551CMN
CSB-BP804551CMN
CSB-MP804551CMN
CSB-EP804551CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP804552CMN
CSB-EP804552CMN
CSB-BP804552CMN
CSB-MP804552CMN
CSB-EP804552CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose import ATP-binding protein RbsA (rbsA), partial CSB-YP804553CMN
CSB-EP804553CMN
CSB-BP804553CMN
CSB-MP804553CMN
CSB-EP804553CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 33 kDa chaperonin (hslO) CSB-YP804554CMN
CSB-EP804554CMN
CSB-BP804554CMN
CSB-MP804554CMN
CSB-EP804554CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit hemorrhagic disease virus Genome polyprotein (ORF1), partial CSB-YP804555RCAY
CSB-EP804555RCAY
CSB-BP804555RCAY
CSB-MP804555RCAY
CSB-EP804555RCAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chorismate synthase (aroC) CSB-YP804556CMN
CSB-EP804556CMN
CSB-BP804556CMN
CSB-MP804556CMN
CSB-EP804556CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L21 (rplU) CSB-YP804557CMN
CSB-EP804557CMN
CSB-BP804557CMN
CSB-MP804557CMN
CSB-EP804557CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP804558CMN
CSB-EP804558CMN
CSB-BP804558CMN
CSB-MP804558CMN
CSB-EP804558CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyguanosinetriphosphate triphosphohydrolase-like protein (CTC_02012) CSB-YP804559CMN
CSB-EP804559CMN
CSB-BP804559CMN
CSB-MP804559CMN
CSB-EP804559CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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