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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium japonicum 3-dehydroquinate synthase (aroB) CSB-YP805161BVW
CSB-EP805161BVW
CSB-BP805161BVW
CSB-MP805161BVW
CSB-EP805161BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Enolase (eno) CSB-YP805162BDU
CSB-EP805162BDU
CSB-BP805162BDU
CSB-MP805162BDU
CSB-EP805162BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Ribonuclease Y (rny), partial CSB-YP805163BDU
CSB-EP805163BDU
CSB-BP805163BDU
CSB-MP805163BDU
CSB-EP805163BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Protein translocase subunit SecA (secA), partial CSB-YP805164BDU
CSB-EP805164BDU
CSB-BP805164BDU
CSB-MP805164BDU
CSB-EP805164BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L27 (rpmA) CSB-YP805165BDU
CSB-EP805165BDU
CSB-BP805165BDU
CSB-MP805165BDU
CSB-EP805165BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP805166BDU
CSB-EP805166BDU
CSB-BP805166BDU
CSB-MP805166BDU
CSB-EP805166BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP805167BDU
CSB-EP805167BDU
CSB-BP805167BDU
CSB-MP805167BDU
CSB-EP805167BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase 2 (kduI2) CSB-YP805168BDU
CSB-EP805168BDU
CSB-BP805168BDU
CSB-MP805168BDU
CSB-EP805168BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP805169BDU
CSB-EP805169BDU
CSB-BP805169BDU
CSB-MP805169BDU
CSB-EP805169BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Redox-sensing transcriptional repressor rex (rex) CSB-YP805170BDU
CSB-EP805170BDU
CSB-BP805170BDU
CSB-MP805170BDU
CSB-EP805170BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron DNA mismatch repair protein mutL (mutL), partial CSB-YP805171BDU
CSB-EP805171BDU
CSB-BP805171BDU
CSB-MP805171BDU
CSB-EP805171BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L32 (rpmF) CSB-YP805172BDU
CSB-EP805172BDU
CSB-BP805172BDU
CSB-MP805172BDU
CSB-EP805172BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP805173BDU
CSB-EP805173BDU
CSB-BP805173BDU
CSB-MP805173BDU
CSB-EP805173BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron D-alanine--D-alanine ligase (ddl) CSB-YP805174BDU
CSB-EP805174BDU
CSB-BP805174BDU
CSB-MP805174BDU
CSB-EP805174BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP805175BDU
CSB-EP805175BDU
CSB-BP805175BDU
CSB-MP805175BDU
CSB-EP805175BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron UPF0145 protein BT_3410 (BT_3410) CSB-YP805176BDU
CSB-EP805176BDU
CSB-BP805176BDU
CSB-MP805176BDU
CSB-EP805176BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Acyl carrier protein (acpP) CSB-YP805177BDU
CSB-EP805177BDU
CSB-BP805177BDU
CSB-MP805177BDU
CSB-EP805177BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase 1 (kduI1) CSB-YP805178BDU
CSB-EP805178BDU
CSB-BP805178BDU
CSB-MP805178BDU
CSB-EP805178BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Alpha-1,3-galactosidase A (glaA), partial CSB-YP805179BDU
CSB-EP805179BDU
CSB-BP805179BDU
CSB-MP805179BDU
CSB-EP805179BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Arginine--tRNA ligase (argS), partial CSB-YP805180BDU
CSB-EP805180BDU
CSB-BP805180BDU
CSB-MP805180BDU
CSB-EP805180BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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