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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant African swine fever virus Protein MGF 300-1L (BA71V-019), partial CSB-YP804580AEJ
CSB-EP804580AEJ
CSB-BP804580AEJ
CSB-MP804580AEJ
CSB-EP804580AEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Structural DNA-binding protein p10 (Ba71V-049) CSB-YP804581AEJ
CSB-EP804581AEJ
CSB-BP804581AEJ
CSB-MP804581AEJ
CSB-EP804581AEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ABC transporter ATP-binding protein CTC_00753 (CTC_00753) CSB-YP804582CMN
CSB-EP804582CMN
CSB-BP804582CMN
CSB-MP804582CMN
CSB-EP804582CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobalamin biosynthesis protein CobD (cobD), partial CSB-YP804583CMN1
CSB-EP804583CMN1
CSB-BP804583CMN1
CSB-MP804583CMN1
CSB-EP804583CMN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized membrane protein KP93L/DP93R (BA71V-002), partial CSB-YP804584AEJ1
CSB-EP804584AEJ1
CSB-BP804584AEJ1
CSB-MP804584AEJ1
CSB-EP804584AEJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus C Outer capsid glycoprotein VP7 CSB-YP804585RHJ
CSB-EP804585RHJ
CSB-BP804585RHJ
CSB-MP804585RHJ
CSB-EP804585RHJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Uncharacterized protein DR2 (DR2L), partial CSB-YP804586HJZ
CSB-EP804586HJZ
CSB-BP804586HJZ
CSB-MP804586HJZ
CSB-EP804586HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD), partial CSB-YP804587CMN
CSB-EP804587CMN
CSB-BP804587CMN
CSB-MP804587CMN
CSB-EP804587CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative K (+)-stimulated pyrophosphate-energized sodium pump (hppA), partial CSB-YP804588CMN
CSB-EP804588CMN
CSB-BP804588CMN
CSB-MP804588CMN
CSB-EP804588CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein translocase subunit SecA (secA), partial CSB-YP804589CMN
CSB-EP804589CMN
CSB-BP804589CMN
CSB-MP804589CMN
CSB-EP804589CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adelaide River virus Non-structural glycoprotein (GNS), partial CSB-YP804590AEAC
CSB-EP804590AEAC
CSB-BP804590AEAC
CSB-MP804590AEAC
CSB-EP804590AEAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Gag-Pol polyprotein (gag-pol), partial CSB-YP804591HME
CSB-EP804591HME
CSB-BP804591HME
CSB-MP804591HME
CSB-EP804591HME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP804592CMN
CSB-EP804592CMN
CSB-BP804592CMN
CSB-MP804592CMN
CSB-EP804592CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose-phosphate pyrophosphokinase (prs) CSB-YP804593CMN
CSB-EP804593CMN
CSB-BP804593CMN
CSB-MP804593CMN
CSB-EP804593CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5'-nucleotidase surE (surE) CSB-YP804594CMN
CSB-EP804594CMN
CSB-BP804594CMN
CSB-MP804594CMN
CSB-EP804594CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0735 ACT domain-containing protein CTC_00116 (CTC_00116) CSB-YP804595CMN
CSB-EP804595CMN
CSB-BP804595CMN
CSB-MP804595CMN
CSB-EP804595CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA modification GTPase MnmE (mnmE) CSB-YP804596CMN
CSB-EP804596CMN
CSB-BP804596CMN
CSB-MP804596CMN
CSB-EP804596CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0133 protein CTC_00074 (CTC_00074) CSB-YP804597CMN
CSB-EP804597CMN
CSB-BP804597CMN
CSB-MP804597CMN
CSB-EP804597CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Co-chaperone protein hscB (hscB) CSB-YP804598BMZ
CSB-EP804598BMZ
CSB-BP804598BMZ
CSB-MP804598BMZ
CSB-EP804598BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized protein bbp_529 (bbp_529) CSB-YP804599BMZ
CSB-EP804599BMZ
CSB-BP804599BMZ
CSB-MP804599BMZ
CSB-EP804599BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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