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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus plantarum Glycogen synthase (glgA) CSB-YP805001LMS
CSB-EP805001LMS
CSB-BP805001LMS
CSB-MP805001LMS
CSB-EP805001LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Chromosomal replication initiator protein DnaA (dnaA) CSB-YP805002LMS
CSB-EP805002LMS
CSB-BP805002LMS
CSB-MP805002LMS
CSB-EP805002LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L11 (rplK) CSB-YP805003CMN
CSB-EP805003CMN
CSB-BP805003CMN
CSB-MP805003CMN
CSB-EP805003CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP805004CMN
CSB-EP805004CMN
CSB-BP805004CMN
CSB-MP805004CMN
CSB-EP805004CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L6 (rplF) CSB-YP805005CMN
CSB-EP805005CMN
CSB-BP805005CMN
CSB-MP805005CMN
CSB-EP805005CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP805006CMN
CSB-EP805006CMN
CSB-BP805006CMN
CSB-MP805006CMN
CSB-EP805006CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylaspartate mutase S chain (mamA) CSB-YP805007CMN
CSB-EP805007CMN
CSB-BP805007CMN
CSB-MP805007CMN
CSB-EP805007CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative sporulation transcription regulator WhiA (whiA) CSB-YP805008CMN
CSB-EP805008CMN
CSB-BP805008CMN
CSB-MP805008CMN
CSB-EP805008CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase (pgi) CSB-YP805009CMN
CSB-EP805009CMN
CSB-BP805009CMN
CSB-MP805009CMN
CSB-EP805009CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP805010CMN
CSB-EP805010CMN
CSB-BP805010CMN
CSB-MP805010CMN
CSB-EP805010CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant V-type ATP synthase alpha chain 2 (atpA2), partial CSB-YP805011CMN
CSB-EP805011CMN
CSB-BP805011CMN
CSB-MP805011CMN
CSB-EP805011CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Valine--tRNA ligase (valS), partial CSB-YP805012CMN
CSB-EP805012CMN
CSB-BP805012CMN
CSB-MP805012CMN
CSB-EP805012CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L20 (rplT) CSB-YP805013CMN
CSB-EP805013CMN
CSB-BP805013CMN
CSB-MP805013CMN
CSB-EP805013CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP805014CMN
CSB-EP805014CMN
CSB-BP805014CMN
CSB-MP805014CMN
CSB-EP805014CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soil-borne wheat mosaic virus 84 kDa readthrough protein (CP-CP2), partial CSB-YP805015SIK
CSB-EP805015SIK
CSB-BP805015SIK
CSB-MP805015SIK
CSB-EP805015SIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate--tRNA ligase (aspS), partial CSB-YP805016CMN
CSB-EP805016CMN
CSB-BP805016CMN
CSB-MP805016CMN
CSB-EP805016CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase obg (obg) CSB-YP805017CMN
CSB-EP805017CMN
CSB-BP805017CMN
CSB-MP805017CMN
CSB-EP805017CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyribose-phosphate aldolase (deoC) CSB-YP805018CMN
CSB-EP805018CMN
CSB-BP805018CMN
CSB-MP805018CMN
CSB-EP805018CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formimidoylglutamase (hutG) CSB-YP805019CMN
CSB-EP805019CMN
CSB-BP805019CMN
CSB-MP805019CMN
CSB-EP805019CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar assembly factor FliW (fliW) CSB-YP805020CMN
CSB-EP805020CMN
CSB-BP805020CMN
CSB-MP805020CMN
CSB-EP805020CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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