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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum Acyl carrier protein (acpP) CSB-YP805755BVW
CSB-EP805755BVW
CSB-BP805755BVW
CSB-MP805755BVW
CSB-EP805755BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum PKHD-type hydroxylase blr3905 (blr3905) CSB-YP805756BVW
CSB-EP805756BVW
CSB-BP805756BVW
CSB-MP805756BVW
CSB-EP805756BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum C4-dicarboxylate transport protein 2 (dctA2), partial CSB-YP805757BVW1
CSB-EP805757BVW1
CSB-BP805757BVW1
CSB-MP805757BVW1
CSB-EP805757BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum FMN-dependent NADH-azoreductase 1 (azoR1) CSB-YP805758BVW
CSB-EP805758BVW
CSB-BP805758BVW
CSB-MP805758BVW
CSB-EP805758BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Formyl-coenzyme A transferase (frc) CSB-YP805759BVW
CSB-EP805759BVW
CSB-BP805759BVW
CSB-MP805759BVW
CSB-EP805759BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0317 protein blr2921 (blr2921) CSB-YP805760BVW
CSB-EP805760BVW
CSB-BP805760BVW
CSB-MP805760BVW
CSB-EP805760BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Chorismate synthase (aroC) CSB-YP805761BVW
CSB-EP805761BVW
CSB-BP805761BVW
CSB-MP805761BVW
CSB-EP805761BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0337 protein bsl2407 (bsl2407) CSB-YP805762BVW
CSB-EP805762BVW
CSB-BP805762BVW
CSB-MP805762BVW
CSB-EP805762BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Nicotinate phosphoribosyltransferase (pncB) CSB-YP805763BVW
CSB-EP805763BVW
CSB-BP805763BVW
CSB-MP805763BVW
CSB-EP805763BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP805764BVW
CSB-EP805764BVW
CSB-BP805764BVW
CSB-MP805764BVW
CSB-EP805764BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Sulfate/thiosulfate import ATP-binding protein CysA (cysA) CSB-YP805765BVW
CSB-EP805765BVW
CSB-BP805765BVW
CSB-MP805765BVW
CSB-EP805765BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Urease accessory protein UreD (ureD) CSB-YP805766BVW
CSB-EP805766BVW
CSB-BP805766BVW
CSB-MP805766BVW
CSB-EP805766BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Histidinol-phosphate aminotransferase 2 (hisC2) CSB-YP805767BVW
CSB-EP805767BVW
CSB-BP805767BVW
CSB-MP805767BVW
CSB-EP805767BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ribose 1,5-bisphosphate phosphokinase PhnN (phnN) CSB-YP805768BVW
CSB-EP805768BVW
CSB-BP805768BVW
CSB-MP805768BVW
CSB-EP805768BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Aliphatic amidase (amiE) CSB-YP805769BVW
CSB-EP805769BVW
CSB-BP805769BVW
CSB-MP805769BVW
CSB-EP805769BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP805770BVW
CSB-EP805770BVW
CSB-BP805770BVW
CSB-MP805770BVW
CSB-EP805770BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable rRNA maturation factor (bll0793) CSB-YP805771BVW
CSB-EP805771BVW
CSB-BP805771BVW
CSB-MP805771BVW
CSB-EP805771BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum tRNA pseudouridine synthase B (truB) CSB-YP805772BVW
CSB-EP805772BVW
CSB-BP805772BVW
CSB-MP805772BVW
CSB-EP805772BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Tryptophan synthase alpha chain (trpA) CSB-YP805773BVW
CSB-EP805773BVW
CSB-BP805773BVW
CSB-MP805773BVW
CSB-EP805773BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Translation initiation factor IF-3 (infC) CSB-YP805774BVW
CSB-EP805774BVW
CSB-BP805774BVW
CSB-MP805774BVW
CSB-EP805774BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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